| 摘要: |
| ［摘要］ 目的 探讨胚胎动力学参数与胚胎发育潜能之间的关系，为筛选发育潜能更好的胚胎提供依据。方法 回顾性分析在该中心接受IVF/ICSI治疗的患者，来源为2PN的胚胎，采用time-lapse技术培养，共165枚。按照卵裂期和囊胚期常规胚胎评分标准进行分组，比较各组间胚胎动力学参数的差异。结果 （1）根据受精后第3天评分分为两组：1～2级胚胎组（n=85）和3～4级胚胎组(n=80)。3～4级胚胎组在tPNa、tPNf和t2等参数比1～2级胚胎组有所延迟，差异无统计学意义（P>0.05）；而在第二次卵裂（t3/t4）上3～4级胚胎组要比1～2级胚胎组有所提前，差异无统计学意义（P>0.05）。（2）根据受精后第5～6天评分分为三组：优质囊胚组（n=20）、可冻囊胚组（n=17）和劣质囊胚组（n=23）。可冻囊胚组tPNa和t2参数较优质囊胚组的发育速度稍微延迟，差异无统计学意义（P>0.05）；劣质囊胚组比优质囊胚组的发育速度显著延迟，差异有统计学意义（P<0.05）。在tSC、tMf/p和tSB参数方面，可冻囊胚组和劣质囊胚组较优质囊胚组的发育速度都显著延迟，三组差异有统计学意义（P<0.05）。结论 t2、tSC、tMf/p和tSB等时间参数可能与囊胚的形成具有相关性，可以用来预测胚胎的发育潜能。 |
| 关键词: 时差成像技术 胚胎发育 妊娠率 胚胎选择 |
| DOI：10.3969/j.issn.1674-3806.2016.09.01 |
| 分类号:R 711 |
| 基金项目:广西卫计委科研课题（编号：Z2013343）；广西科学研究与技术开发计划项目（编号：桂科攻1140003B-66） |
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| Preliminary study of the relationship between the kinetic parameters and the developmental potential of embryo |
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WANG Shi-kai, XUE Lin-tao, HE Bing, et al.
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Reproductive and Genetic Center, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the relationship between the kinetic parameters and the developmental potential of embryos, and to provide the evidences for the selection of better developmental potential embryos.Methods A retrospective analysis of 165 2PN embryos was performed in our center using time-lapse technology. The embryos were divided into different groups according to the criteria of the embryo scores and the kinetic parameters were compared among the groups.Results （1）The embryos were divided into two groups according D3 embryo scores: grade 1～2 group(n=85) and grade 3～4 group(n=80). The development of the grade 3～4 group in the parameters of tPNa, tPNf and t2 was delayed than that of the grade 1～2 group, but without a significant difference between the two groups(P>0.05). The time of t3/t4 in the grade 3～4 group was less than that in the grade 1～2 group, but without a significant difference(P>0.05). （2）The embryos were divided into three groups according D5 embryo scores: high quality blastocysts group(n=20), frozen blastocysts group(n=17) and poor quality blastocysts group(n=23). The parameters of tPNa and t2 in the frozen blastocyst group were slightly delayed than those in the high quality blastocysts group(P>0.05). The time of tPNa and t2 was significantly delayed in the poor quality blastocysts group than that in the high quality blastocysts group(P<0.05). There were significant differences among the three groups in the parameters of tSC, tMf/p and tSB(P<0.05).Conclusion The time parameters of t2, tSC, tMf/p and tSB may be related to the formation of the blastocyst, which can be used to predict the developmental potential of the embryos. |
| Key words: Time-lapse Embryo development Pregnancy rate Embryo selection |
