| 摘要: |
| ［摘要］ 目的 探讨石菖蒲对抑郁大鼠脑内神经递质含量及行为学的影响。方法 将30只SD大鼠随机分为空白组、模型组、氟西汀组［1.8 mg/(kg·d)］、石菖蒲低剂量组［5 ml/(kg·d)］、石菖蒲中剂量组［10 ml/(kg·d)］和石菖蒲高剂量组［20 ml/(kg·d)］，每组5只。除空白组外，其余5组予以慢性、不可预见的温和刺激（CUMS）建造抑郁大鼠模型。通过强迫游泳试验(FST)、旷场试验(OFT)、高架十字迷宫试验(EPM)比较各组抑郁症状情况。比较各组大鼠脑组织多巴胺（DA）和5-羟色胺（5-HT）水平。结果 行为学实验结果显示，模型组游泳不动时间高于空白组，OFT得分、开放臂停留时间（OT）/两臂停留总时间（TT）低于空白组，差异有统计学意义（P<0.05）。模型组脑组织DA、5-HT水平显著低于空白组（P<0.05）。石菖蒲高剂量组、石菖蒲中剂量组、石菖蒲低剂量组、氟西汀组OFT得分、游泳不动时间、OT/TT较模型组改善，差异有统计学意义（P<0.05）。随着石菖蒲剂量的升高，大鼠脑组织DA、5-HT水平均呈上升趋势。石菖蒲高剂量组和氟西汀组脑组织DA、5-HT水平均显著高于模型组（P<0.05），而与空白组比较差异无统计学意义（P>0.05）。结论 石菖蒲通过提高脑组织中的神经递质水平发挥抗抑郁作用。 |
| 关键词: 石菖蒲 抑郁症 神经递质 行为学 |
| DOI：10.3969/j.issn.1674-3806.2021.12.07 |
| 分类号:R 749.4 |
| 基金项目:黑龙江省大学生创新创业训练计划项目（编号：201911230005） |
|
| Effects of Acorus calamus on neurotransmitter content in the brain of depression rats and their ethology |
|
FU Jia, XUN Chen-xi, LI Shuang, et al.
|
|
School of Mental Health, Qiqihar Medical College, Heilongjiang 161000, China
|
| Abstract: |
| ［Abstract］ Objective To explore the effects of Acorus calamus on neurotransmitter content in the brain of depression rats and their ethology. Methods Thirty Sprague-Dawley(SD) rats were randomly divided into blank group, model group, fluoxetine group［1.8 mg/(kg·d)］, Acorus calamus low-dose group［5 ml/(kg·d)］, Acorus calamus middle-dose group［10 ml/(kg·d)］ and Acorus calamus high-dose group［20 ml/(kg·d)］, with 5 rats in each group. Except for the blank group, the other 5 groups were given chronic unpredictable mild stress(CUMS) to establish depression rat models. Forced swimming test(FST), open field test(OFT) and elevated plus-maze test(EPM) were used to compare the depressive symptoms among the 6 groups. The levels of dopamine(DA) and 5-hydroxytryptamine(5-HT) in brain tissues of rats were compared among the 6 groups. Results The results of behavioral experiments showed that swimming immobility time of the model group was longer than that of the blank group, and that the OFT score and open-arm time(OT)/ total time(TT) of the model group were lower than those of the blank group, and the differences were statistically significant(P<0.05). The levels of DA and 5-HT in brain tissues in the model group were significantly lower than those in the blank group(P<0.05). The OFT score, swimming immobility time and OT/TT in the Acorus calamus high-dose group, Acorus calamus middle-dose group, Acorus calamus low-dose group, and the fluoxetine group improved compared with those in the model group, and the differences were statistically significant(P<0.05). With the increase of the dose of Acorus calamus, the levels of DA and 5-HT in brain tissues of rats showed an upward trend. The levels of DA and 5-HT in brain tissues in the Acorus calamus high-dose group and the fluoxetine group were significantly higher than those in the model group(P<0.05). However, compared with those in the blank group, the levels of DA and 5-HT in the Acorus calamus high-dose group and the fluoxetine group were not significantly different(P>0.05). Conclusion Acorus calamus exerts an antidepressant effect by increasing the level of neurotransmitters in the brain tissues. |
| Key words: Acorus calamus Depression Neurotransmitter Ethology |
