| 摘要: |
| ［摘要］ 目的 探讨结直肠癌(CRC)组织中孤儿受体G蛋白偶联受体15(GPR15)和高迁移率族蛋白组A1(HMGA1)的表达情况及其临床预后价值。方法 选取2019年1月至2020年1月于扬州大学附属医院接受根治性手术治疗的89例CRC患者，收集患者术中的CRC癌组织和癌旁组织。采用实时荧光定量聚合酶链式反应及免疫组化染色法检测组织中GPR15和HMGA1的表达情况，分析CRC癌组织中GPR15及HMGA1表达与患者临床病理特征的关联性。采用Kaplan-Meier法分析CRC癌组织中GPR15、HMGA1表达情况与患者生存预后的关联性。采用Cox回归分析CRC患者预后的影响因素。结果 CRC癌组织中GPR15、HMGA1 mRNA相对表达量显著高于癌旁组织(P<0.05)。CRC癌组织中GPR15、HMGA1蛋白的阳性表达率显著高于癌旁组织(P<0.05)。TNM分期Ⅲ期、低分化程度及合并淋巴结转移的CRC癌组织中GPR15、HMGA1阳性表达率分别高于Ⅰ～Ⅱ期、高中分化程度及无淋巴结转移的CRC癌组织，差异有统计学意义(P<0.05)。GPR15阴性组生存预后显著优于阳性组（log-rank检验： χ2=9.124，P=0.003）；HMGA1阴性组生存预后显著优于阳性组（log-rank检验： χ2=10.140，P=0.001）。肿瘤TNM分期Ⅲ期、低分化程度、合并淋巴结转移、GPR15阳性、HMGA1阳性是促进CRC患者不良预后的独立危险因素（P<0.05）。结论 CRC癌组织中GPR15和HMGA1的表达水平升高，两者与CRC患者不良临床病理特征密切相关，可作为新的评估CRC患者预后的肿瘤标志物。 |
| 关键词: 结直肠癌 孤儿受体G蛋白偶联受体15 高迁移率族蛋白组A1 预后 |
| DOI：10.3969/j.issn.1674-3806.2023.12.10 |
| 分类号:R 735.34 |
| 基金项目:江苏省卫生健康委科研项目（编号：M2020068） |
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| A study on the expressions of GPR15 and HMGA1 in colorectal cancer tissues and their clinical prognostic value |
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WANG Peng-hui, HU Jie, PAN Rong-yan
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Department of Laboratory, Affiliated Hospital of Yangzhou University, Jiangsu 225000, China
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| Abstract: |
| ［Abstract］ Objective To investigate the expressions of orphan receptor G protein coupled receptor 15(GPR15) and high mobility group proteome A1(HMGA1) in colorectal cancer(CRC) tissues and their clinical prognostic value. Methods Eighty-nine CRC patients who underwent radical surgery in Affiliated Hospital of Yangzhou University from January 2019 to January 2020 were selected, and their intraoperative CRC tissues and the cancer-adjacent tissues were collected. Quantitative real-time polymerase chain reaction(qRT-PCR) and immunohistochemistry staining were used to detect the expressions of GPR15 and HMGA1 in the tissues. The correlation between the expressions of GPR15 and HMGA1 in the CRC tissues and the clinicopathological characteristics of the patients was analyzed. The Kaplan-Meier method was used to analyze the correlation between the expressions of GPR15 and HMGA1 in the CRC tissues and the patients′ survival prognosis. Cox regression was used to analyze the influencing factors of prognosis in the CRC patients. Results The relative expression levels of GPR15 and HMGA1 mRNA in the CRC tissues were significantly higher than those in the cancer-adjacent tissues(P<0.05). The positive expression rates of GPR15 and HMGA1 proteins in the CRC tissues were significantly higher than those in the cancer-adjacent tissues(P<0.05). The positive expression rates of GPR15 and HMGA1 in the CRC tissues with TNM stage Ⅲ, low differentiation, and combined lymph node metastasis were higher than those in the CRC tissues with TNM stage Ⅰ-Ⅱ, high differentiation and non-lymph node metastasis, respectively(P<0.05). The survival prognosis of the GPR15 negative group was significantly better than that of the GPR15 positive group(log-rank test: χ2=9.124, P=0.003). The survival prognosis of the HMGA1 negative group was significantly better than that of the HMGA1 positive group(log-rank test: χ2=10.140, P=0.001). Tumor TNM stage Ⅲ, low differentiation, combined lymph node metastasis, GPR15 positive and HMGA1 positive were the independent risk factors promoting poor prognosis of the CRC patients(P<0.05). Conclusion The expression levels of GPR15 and HMGA1 are increased in CRC tissues, which are closely related to adverse clinicopathological characteristics of CRC patients. GPR15 and HMGA1 can be used as new tumor markers to evaluate the prognosis of the CRC patients. |
| Key words: Colorectal cancer Orphan receptor G protein coupled receptor 15(GPR15) High mobility group proteome A1(HMGA1) Prognosis |
