| 摘要: |
| ［摘要］ 目的 探究牙髓干细胞条件培养基(DPSCs-CM)联合层状双金属氢氧化物（LDH）纳米材料对光老化HaCaT细胞的作用。方法 制备DPSCs-CM并合成LDH纳米材料。设置对照组［无预处理，无中波紫外线(UVB)照射］、模型组（无预处理，予UVB照射）、DPSCs-CM组（用含DPSCs-CM的DMEM完全培养基预处理24 h后进行UVB照射）、LDH组（用含LDH的DMEM完全培养基预处理24 h后进行UVB照射）和DPSCs-CM+LDH组（用含有等比混合的DPSCs-CM和LDH的DMEM完全培养基预处理24 h后进行UVB照射）。检测各组HaCaT细胞的丙二醛（MDA）含量、超氧化物歧化酶（SOD）活力以及活性氧（ROS）水平。采用细胞划痕实验检测各组HaCaT细胞的迁移能力。采用Western blot法检测各组HaCaT细胞中p53蛋白和p16蛋白的表达水平。结果 LDH纳米材料的平均粒径为65.52 nm，分散度系数（PDI）为0.109，Zeta电位为38.1 mV，表明LDH纳米材料的细胞毒性较低，纳米粒子粒径分布均一，分散性和稳定性良好。与模型组相比，DPSCs-CM组、LDH组和DPSCs-CM+LDH组HaCaT细胞的ROS水平和MDA含量降低，SOD活力升高，细胞迁移能力提高，细胞内p53蛋白和p16蛋白表达水平降低，差异有统计学意义（P<0.05），且以DPSCs-CM+LDH组的干预效果最佳。结论 DPSCs-CM联合LDH纳米材料预处理可有效抗HaCaT细胞光老化，其效果优于单一DPSCs-CM和LDH纳米材料干预，为皮肤光老化治疗方法的研发提供了参考。 |
| 关键词: 牙髓干细胞条件培养基 层状双金属氢氧化物 纳米材料 中波紫外线 HaCaT细胞 光老化 |
| DOI：10.3969/j.issn.1674-3806.2024.09.08 |
| 分类号:R 751.05 |
| 基金项目:国家自然科学基金资助项目（编号：82260614） |
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| A study on the anti-photoaging effect of dental pulp stem cells-conditioned medium combined with LDH nanomaterials on HaCaT cells |
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QIU Liuliu, XIE Shenxia, YANG Xiaomei, LU Xiaoling
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College & Hospital of Stomatology, Guangxi Medical University, Guangxi Zhuang Autonomous Region Nanobody Engineering Research Center, Guangxi Key Laboratory for Nanobody Research, Guangxi Medical University Nanobody Research Center, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the effect of dental pulp stem cells-conditioned medium(DPSCs-CM) combined with layered double hydroxide(LDH) nanomaterials on photoaging HaCaT cells. Methods DPSCs-CM was prepared and LDH nanomaterials were synthesized. The control group［no pretreatment, no exposure to ultraviolet B(UVB)］, model group(no pretreatment, exposure to UVB), DPSCs-CM group(undergoing UVB irradiation after 24-hour pretreatment with DMEM complete medium containing DPSCs-CM), LDH group(undergoing UVB irradiation after 24-hour pretreatment with DMEM complete medium containing LDH) and DPSCs-CM+LDH group(undergoing UVB irradiation after 24-hour pretreatment with DMEM complete medium containing DPSCs-CM and LDH mixed in equal proportion) were set up. The content of malondialdehyde(MDA), the activity of superoxide dismutase(SOD) and the level of reactive oxygen species(ROS) of HaCaT cells were detected in each group. The migration ability of HaCaT cells in each group was detected by using scratch wound healing assay. The expression levels of p53 protein and p16 protein of HaCaT cells in each group were detected by using Western blot method. Results The average particle size of the LDH nanomaterials was 65.52 nm, with a polydispersity index(PDI) of 0.109 and a Zeta potential of 38.1 mV, indicating that LDH nanomaterials had low cytotoxicity, uniform particle size distribution, and good dispersion and stability. Compared with the model group, the DPSCs-CM group, LDH group and DPSCs-CM+LDH group had the low level of ROS and low content of MDA, high activity of SOD, the enhanced migration ability of HaCaT cells and reduced expression levels of intracellular p53 protein and p16 protein, and the differences were statistically significant(P<0.05). The intervention effect of the DPSCs-CM+LDH group was the best. Conclusion DPSCs-CM combined with LDH nanomaterials can effectively prevent photoaging of HaCaT cells, and its effect is better than that of single DPSCs-CM intervention or LDH nanomaterials intervention, which provides a reference for the research and development of skin photoaging treatment methods. |
| Key words: Dental pulp stem cells-conditioned medium(DPSCs-CM) Layered double hydroxide(LDH) Nanomaterial Ultraviolet B(UVB) HaCaT cell Photoaging |
