| 摘要: |
| ［摘要］ 目的 分析单中心山东汉族肾移植受者HLA-A、HLA-B、HLA-C、HLA-DRB1、HLA-DQB1等位基因分布及单体型多态性特点，获取肾移植人群人类白细胞抗原（HLA）遗传学参数。方法 选择2019年6月至2022年12月于青岛大学附属医院进行HLA-A、HLA-B、HLA-C、HLA-DRB1、HLA-DQB1等位基因检测的1 470例肾移植受者。采用序列特异性寡核苷酸探针-聚合酶链反应法进行HLA等位基因检测。采用直接计数法计算HLA等位基因频率，对各等位基因位点进行Hardy-Weinberg平衡检验。采用Arlequin 3.5.2.2软件计算常见单体型频率。结果 1 470例患者中共检测到216个等位基因，其中HLA-A、HLA-B、HLA-C、HLA-DRB1、HLA-DQB1分别检出等位基因数量为36、86、33、40、21个。HLA-A、HLA-B、HLA-C等位基因的观察值与期望值比较差异无统计学意义（P>0.05），HLA-DRB1和HLA-DQB1等位基因的观察值与期望值比较差异有统计学意义（P<0.05）。HLA-A～HLA-B单体型452对，HLA-B～HLA-C单体型219对，HLA-A～HLA-B～HLA-C单体型666对，HLA-B～HLA-DRB1单体型551对，HLA-DRB1～HLA-DQB1单体型91对，HLA-A～HLA-B～HLA-C～HLA-DRB1单体型1 367对，HLA-A～HLA-B～HLA-C～HLA-DRB1～HLA-DQB1单体型1 434对。HLA-A*30:01～HLA-B*13:02～HLA-C*06:02～HLA-DRB1*07:01～HLA-DQB1*02:02单体型频率最高。结论 山东汉族肾移植受者中HLA-A*30:01～HLA-B*13:02～HLA-C*06:02～HLA-DRB1*07:01～HLA-DQB1*02:02单体型频率最高，该研究为供者等位基因检索以及受者人群遗传学研究提供了参考。 |
| 关键词: 人类白细胞抗原 肾移植 等位基因 单体型 |
| DOI：10.3969/j.issn.1674-3806.2025.01.13 |
| 分类号:R 699.2 |
| 基金项目:山东省输血协会科研基金项目（编号：2024-sdsx09） |
|
| Analysis on the allelic distribution and haplotype polymorphism of HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQB1 in the renal transplantation recipients of a single-center Han population in Shandong |
|
SHAO Changfeng1, KONG Yirong2, WANG Liqin1, WANG Rong1, JU Qiang1, CHEN Shumin1, WANG Haiyan1
|
|
1.Department of Blood Transfusion, the Affiliated Hospital of Qingdao University, Qingdao 266003, China; 2.Department of Clinical Laboratory, Qingdao Municipal Hospital, Qingdao 266003, China
|
| Abstract: |
| ［Abstract］ Objective To analyze characteristics of the allelic distribution and haplotype polymorphism of HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DQB1 in the renal transplantation recipients of a single-center Han population in Shandong, and to obtain human leukocyte antigen(HLA) genetic parameters of the kidney transplant population. Methods A total of 1 470 renal transplantation recipients who underwent allele detection of HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1 in the Affiliated Hospital of Qingdao University from June 2019 to December 2022 were selected. The method of polymerase chain reaction-sequence specific oligonucleotide probe(PCR-SSOP) was used for allele detection of HLA. The frequency of HLA alleles was calculated by using direct counting method, and Hardy-Weinberg equilibrium test was performed for the loci of each allele. The common haplotype frequencies were calculated by using Arlequin 3.5.2.2 software. Results A total of 216 alleles were detected in the 1 470 patients, among which 36, 86, 33, 40 and 21 alleles were detected in HLA-A, HLA-B, HLA-C, HLA-DRB1 and HLA-DQB1, respectively. There were no significant differences between the observed values of HLA-A, HLA-B and HLA-C alleles and the expected values of HLA-A, HLA-B and HLA-C alleles(P>0.05). There were significant differences between the observed values of HLA-DRB1 and HLA-DQB1 alleles and the expected values of HLA-DRB1 and HLA-DQB1 alleles(P<0.05). There were a total of 452 haplotype pairs of HLA-A-HLA-B, 219 haplotype pairs of HLA-B-HLA-C, 666 haplotype pairs of HLA-A-HLA-B-HLA-C, 551 haplotype pairs of HLA-B-HLA-DRB1, 91 haplotype pairs of HLA-DRB1-HLA-DQB1, 1 367 haplotype pairs of HLA-A-HLA-B-HLA-C-HLA-DRB1, and 1 434 haplotype pairs of HLA-A-HLA-B-HLA-C-HLA-DRB1-HLA-DQB1. HLA-A*30:01-HLA-B*13:02-HLA-C*06:02-HLA-DRB1*07:01-HLA-DQB1*02:02 haplotype frequency was the highest. Conclusion HLA-A*30:01-HLA-B*13:02-HLA-C*06:02-HLA-DRB1*07:01-HLA-DQB1*02:02 haplotype frequency is the highest in the renal transplantation recipients of the Han population in Shandong. This study provides a reference for donor allele search and recipient population genetics research. |
| Key words: Human leukocyte antigen(HLA) Renal transplantation Allele Haplotype |
