| 摘要: |
| ［摘要］ 目的 分析非小细胞肺癌（NSCLC）患者组织中前病毒插入序列2（PIM2）和果糖-2，6-二磷酸酶异构体3（PFKFB3）表达情况与临床病理特征和生存预后的关联性。方法 选取2020年1月至2021年3月广元市第一人民医院收治的98例NSCLC患者。收集患者的临床资料。术中取NSCLC组织及癌旁组织（距癌组织>3 cm），通过免疫组化染色检测PIM2、PFKFB3表达情况。术后对患者进行3年随访，记录患者的生存情况。采用Cox回归分析影响NSCLC患者生存预后的因素。采用Kaplan-Meier法绘制生存曲线，组间比较采用log-rank检验。结果 PIM2、PFKFB3在NSCLC组织中阳性表达率显著高于癌旁组织（71.43% vs 14.29%，68.73% vs 16.33%；P<0.05）。PIM2、PFKFB3表达情况与临床分期、淋巴结转移情况、分化程度有关（P<0.05），与年龄、性别、肿瘤直径、病理类型无关联（P>0.05）。多因素Cox回归分析结果显示，临床分期为Ⅲ～Ⅳ期、淋巴结转移、PIM2阳性表达、PFKFB3阳性表达是NSCLC患者发生死亡的独立危险因素（P<0.05）。98例NSCLC患者的3年总生存率为54.08%。PIM2及PFKFB3阴性表达患者的生存预后优于阳性表达患者（log-rank检验： χ2=5.777，P=0.016； χ2=5.422，P=0.020）。结论 PIM2、PFKFB3阳性表达与患者不良临床病理特征及不良预后有关。 |
| 关键词: 前病毒插入序列2 果糖-2，6-二磷酸酶异构体3 非小细胞肺癌 临床病理特征 |
| DOI：10.3969/j.issn.1674-3806.2025.06.14 |
| 分类号:R 734.2 |
| 基金项目:四川省科技厅课题（编号：2022NSFSC0725） |
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| Analysis on expressions of PIM2 and PFKFB3 in tissues of patients with non-small cell lung cancer and association of these expressions with clinicopathological features and survival prognosis |
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WEI Jiali1, HUANG Qinrong1, YANG Jinping2, HUANG Na3, YANG Li3
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1.Department of Respiratory and Critical Care Medicine, the First People′s Hospital of Guangyuan, Guangyuan 628000, China; 2.Department of Oncology, the First People′s Hospital of Guangyuan, Guangyuan 628000, China; 3.Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Chengdu Medical College, Chengdu 610500, China
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| Abstract: |
| ［Abstract］ Objective To analyze the expressions of proviral integration site for Moloney murine leukemia virus 2(PIM2) and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3(PFKFB3) in tissues of patients with non-small cell lung cancer(NSCLC) and the association of these expressions with clinicopathological features and survival prognosis. Methods A total of 98 patients with NSCLC who were admitted to the First People′s Hospital of Guangyuan from January 2020 to March 2021 were selected. The clinical data of the patients were collected. During the operation, NSCLC tissues and pericarcinomatous tissues(>3 cm away from the cancer tissues) were collected. The expressions of PIM2 and PFKFB3 were detected by immunohistochemical staining. The patients were followed up for 3 years after the operation. The survival status of the patients was recorded. Cox regression was used to analyze the factors influencing the survival prognosis of the NSCLC patients. The survival curve was plotted using Kaplan-Meier method, and log-rank test was used for inter-group comparison. Results The positive expression rates of PIM2 and PFKFB3 in NSCLC tissues were significantly higher than those in pericarcinomatous tissues(71.43% vs 14.29%, 68.73% vs 16.33%; P<0.05). The expressions of PIM2 and PFKFB3 were related to clinical stage, lymph node metastasis and degree of differentiation(P<0.05), but were not associated with age, gender, tumor diameter and pathological type(P>0.05). The results of multivariate Cox regression analysis showed that clinical stages Ⅲ and Ⅳ, lymph node metastasis, positive expression of PIM2 and positive expression of PFKFB3 were independent risk factors for death in the NSCLC patients(P<0.05). The 3-year overall survival rate of the 98 NSCLC patients was 54.08%. The survival prognosis of the patients with negative expressions of PIM2 and PFKFB3 was better than that of the patients with positive expressions of PIM2 and PFKFB3(log-rank test: χ2=5.777, P=0.016; χ2=5.422, P=0.020). Conclusion Positive expressions of PIM2 and PFKFB3 are associated with the adverse clinicopathological features and poor prognosis of the patients. |
| Key words: Proviral integration site for Moloney murine leukemia virus 2(PIM2) 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3(PFKFB3) Non-small cell lung cancer(NSCLC) Clinicopathological features |
