| 摘要: |
| ［摘要］ 目的 探讨载锶纳米管化纯钛种植体对牙槽骨缺损的修复作用。方法 选择30只3月龄SD大鼠进行实验，随机分为空白组、纳米管组和载锶纳米管组，每组10只。采用纯钛制作无螺纹光滑圆柱形种植体（直径4.5 mm，长度8 mm）。三组种植体处理：空白组，常规抛光后不做处理；纳米管组，使用质量分数为0.5%的氢氟酸溶液，以石墨为阴极，种植体为阳极，经20 V电压阳极氧化处理1 h；载锶纳米管组，在纳米管组的制作基础上采用0.02 mol/L氢氧化锶溶液200 ℃密封水浴处理1 h。拔除大鼠左侧下颌骨切牙，用生理盐水冲洗预备牙槽，种植体放置在距牙龈边缘约8 mm深度的牙槽窝中，大约在第一磨牙根位置。然后用5-0可吸收缝线缝合伤口。每组分别在术后第4周和术后第12周处死5只大鼠，取下颌骨标本进行检测。使用电镜内置能谱分析工具Energy Dispersive Spectrometer分析种植体表面元素组成。使用原子力显微镜测量种植体表面粗糙度。通过显微CT、Masson染色和免疫组化染色进行组织学评价。结果 与空白组相比，纳米管组和载锶纳米管组种植体表面钛（Ti）含量减少，氧（O）、氟（F）含量增加。载锶纳米管组种植体表面氟（F）含量高于纳米管组。与空白组相比，纳米管组和载锶纳米管组种植体表面粗糙度显著增加（P<0.05），且载锶纳米管组表面粗糙度较纳米管组更大（P<0.05）。在术后第4周，纳米管组和载锶纳米管组骨体积/总体积（BV/TV）、骨表面积/骨体积（BS/BV）、骨小梁厚度（Tb.Th）较空白组显著提高（P<0.05）。在术后第12周，载锶纳米管组BV/TV、BS/BV、Tb.Th较空白组和纳米管组显著提高（P<0.05），而纳米管组与空白组比较差异无统计学意义（P>0.05）。在术后第4周和第12周，三组骨小梁数量（Tb.N）和骨小梁间隙（Tb.Sp）比较差异无统计学意义（P>0.05）。Masson染色结果显示，载锶纳米管组的缺损区域周围形成了大量且厚度较大的成熟骨组织，而纳米管组和空白组的骨量较少，且新骨的形成缺乏完整性。在术后第12周，纳米管组和载锶纳米管组大鼠骨缺损部位桥骨蛋白（OPN）、骨形态发生蛋白2（BMP2）阳性染色面积较空白组增加，且载锶纳米管组OPN、BMP2阳性染色面积大于纳米管组，差异有统计学意义（P<0.05）。结论 载锶纳米管化纯钛种植体可以促进牙槽骨缺损的修复。 |
| 关键词: 锶 纳米管 钛 牙种植体 牙槽骨缺损 |
| DOI：10.3969/j.issn.1674-3806.2025.07.11 |
| 分类号:R 783.2 |
| 基金项目:河北省自然科学基金资助项目（编号：H2020109157）；邯郸市科学技术研究与发展计划项目（编号：23422083196） |
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| A study on restorative effect of strontium-loaded nanotube-based pure titanium implants on alveolar bone defects |
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HE Jiahuan1, SONG Liye2, CHENG Bingkun3, TIAN Xiaomei1, LIU Yingqi1
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1.Department of General Stomatology, Handan Central Hospital, Handan 056000, China; 2.Department of Stomatology, Affiliated Hospital of Hebei University of Engineering, Handan 056000, China; 3.Department of Oral and Maxillofacial Surgery, Handan Central Hospital, Handan 056000, China
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| Abstract: |
| ［Abstract］ Objective To explore the restorative effect of strontium-loaded nanotube-based pure titanium(Ti) implants on alveolar bone defects. Methods Thirty 3-month-old Sprague-Dawley(SD) rats were selected for experiment and were randomly divided into blank group, nanotube group and strontium-loaded nanotube group, with 10 rats in each group. Smooth cylindrical implants without screw threads(with a diameter of 4.5 mm and a length of 8 mm) were made of pure Ti. The implants in the three groups were treated differently. For the implants in the blank group, no treatment was given after conventional polishing. The implants of the nanotube group were treated using hydrofluoric acid solution with a mass fraction of 0.5%, with graphite as the cathode and the implants as the anode, and anodic oxidation treatment at a voltage of 20 V for 1 hour. The implants of the strontium-loaded nanotube group were treated with 0.02 mol/L strontium hydroxide solution in a sealed water bath at 200 ℃ for 1 hour on the basis of the treatment of the nanotube group. The incisors of the left mandible of the rats were extracted, and the prepared alveolar sockets were rinsed with normal saline. The implants were placed in the alveolar sockets about 8 mm deep from the edge of the gingiva, approximately at the position of the root of the first molar. Then, the wound was sutured using 5-0 absorbable sutures. Five rats in each group were sacrificed at the 4th and 12th weeks after operation, respectively, and mandibular specimens were taken for detection. The elemental composition of the implant surface was analyzed using an energy spectrum analysis tool of Energy Dispersive Spectrometer built in the electron microscope. The surface roughness of the implants was detected by using atomic force microscopy. Micro-computed tomography, Masson staining and immunohistochemistry staining were used for histological evaluation. Results Compared with those in the blank group, the Ti content detected on the surface of the implants was decreased, while the oxygen(O) content and fluorine(F) content detected on the surface of the implants were increased in the nanotube group and the strontium-loaded nanotube group. The F content detected on the surface of the implants in the strontium-loaded nanotube group was higher than that in the nanotube group. Compared with that in the blank group, the surface roughness of the implants in the nanotube group and the strontium-loaded nanotube group was increased significantly(P<0.05), and the surface roughness of the strontium-loaded nanotube group was greater than that of the nanotube group(P<0.05). Compared with those in the blank group, bone volume/total volume(BV/TV), bone surface/bone volume(BS/BV) and trabecular thickness(Tb.Th) in the nanotube group and the strontium-loaded nanotube group were significantly increased at the 4th week after operation(P<0.05). At the 12th week after operation, compared with those in the blank group and the nanotube group, BV/TV, BS/BV and Tb.Th in the strontium-loaded nanotube group were significantly increased, while there were no statistically significant differences in BV/TV, BS/BV and Tb.Th between the nanotube group and the blank group(P>0.05). At the 4th and 12th weeks after operation, there were no statistically significant differences in trabecular number(Tb.N) and trabecular spacing(Tb.Sp) among the three groups(P>0.05). The Masson staining results showed that a large amount of thick mature bone tissues were formed around the bone defect area of the strontium-loaded nanotube group, while the nanotube group and the blank group had less bone mass and lacked integrity in the formation of new bones. At the 12th week after operation, compared with those in the blank group, the positive staining areas of osteopontin(OPN) and bone morphogenetic protein 2(BMP2) in the bone defect site of the rats in the nanotube group and the strontium-loaded nanotube group were increased, and the positive staining areas of OPN and BMP2 in the bone defect site of the rats in the strontium-loaded nanotube group were larger than those in the nanotube group, with statistically significant differences(P<0.05). Conclusion Strontium-loaded nanotube-based pure Ti implants can promote the restorations in alveolar bone defects. |
| Key words: Strontium Nanotubes Titanium(Ti) Dental implants Alveolar bone defects |
