| 摘要: |
| ［摘要］ 目的 探讨延时成像技术在监测胚胎直接分裂（DC）及评估胚胎发育潜能中的应用价值。方法 回顾性分析2020年7月至2024年2月在广西壮族自治区人民医院生殖医学与遗传中心接受体外受精（IVF）或卵胞浆内单精子注射（ICSI）助孕治疗患者的临床资料，共601个治疗周期，采用延时成像技术监测胚胎的发育过程。根据胚胎分裂模式分为正常分裂组和DC组。根据DC发生时期进一步将DC组分为DC1组（受精卵在第1次分裂时期发生DC）、DC2组（受精卵在第2次分裂时期发生DC）和DC3组（受精卵在第3次分裂时期发生DC）。比较各组胚胎发育潜能指标和妊娠结局指标。结果 延时成像监测结果显示胚胎DC发生率为16.67%（822/4 932）。DC组D3胚胎平均卵裂球数显著少于正常分裂组，D3优质胚胎率、D6囊胚形成率、D6优质囊胚形成率和D6可冻囊胚形成率显著低于正常分裂组（P<0.05）。DC1组和DC2组的D3优质胚胎率显著低于正常分裂组（P<0.05）；DC1组的D3优质胚胎率显著低于DC2组和DC3组（P<0.05）。DC1组和DC2组的D6囊胚形成率显著低于正常分裂组（P<0.05）；DC1组的D6囊胚形成率显著低于DC2组和DC3组（P<0.05）。DC1组、DC2组和DC3组的D6优质囊胚形成率显著低于正常分裂组（P<0.05）；DC1组的D6优质囊胚形成率显著低于DC2组和DC3组（P<0.05）。与正常分裂组相比，DC1组和DC2/3组的生化妊娠率、临床妊娠率、种植率以及活产率均显著降低（P<0.05）。结论 DC会降低胚胎发育潜能，其发生时期越早对胚胎发育的负面影响越大。延时成像技术是监测胚胎发生DC及评估胚胎发育潜能的有效方法。 |
| 关键词: 延时成像 直接分裂 胚胎发育 妊娠结局 |
| DOI：10.3969/j.issn.1674-3806.2025.10.12 |
| 分类号:R 714.5 |
| 基金项目:广西自然科学基金项目（编号：2024GXNSFDA010006，2025GXNSFAA069917） |
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| Study on application of time-lapse imaging technology in monitoring embryonic direct cleavage and evaluating embryonic developmental potential |
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LIU Haifang1, PAN Yongyi1, WANG Shikai2, ZHANG Xiaohui2, WEI Pingpin2, LI Zhengda2, MAO Xianbao2, CHEN Liangshi2, XUE Lintao1,2
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1.Guilin Medical University, Guilin 541001, China; 2.Reproductive Medicine and Genetics Center, the People′s Hospital of Guangxi Zhuang Autonomous Region(Guangxi Academy of Medical Sciences), Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the application value of time-lapse imaging technology in monitoring embryonic direct cleavage(DC) and evaluating embryonic developmental potential. Methods The clinical data of patients who received in vitro fertilization(IVF) or intracytoplasmic sperm injection(ICSI) treatment in Reproductive Medicine and Genetics Center, the People′s Hospital of Guangxi Zhuang Autonomous Region from July 2020 to February 2024 were retrospectively analyzed, with a total of 601 treatment cycles. The development process of embryos was monitored by using time-lapse imaging technology. According to the embryonic cleavage patterns, the embryos were divided into normal cleavage group and DC group. According to the periods of DC, the DC group was further divided into DC1 group(DC occurred in the first division of the fertilized eggs), DC2 group(DC occurred in the second division of the fertilized eggs) and DC3 group(DC occurred in the third division of the fertilized eggs). The indicators of embryonic developmental potential and pregnancy outcomes were compared among the groups. Results The monitoring results of time-lapse imaging showed that the incidence of DC in the embryos was 16.67%(822/4 932). The mean of blastomeres of D3 embryos in the DC group was significantly less than that in the normal cleavage group, and the rates of D3 high-quality embryos, D6 blastocysts formation, D6 high-quality blastocyst formation and D6 freezable blastocyst formation in the DC group were significantly lower than those in the normal cleavage group(P<0.05). The D3 high-quality embryo rates in the DC1 group and the DC2 group were significantly lower than those in the normal cleavage group(P<0.05). The D3 high-quality embryo rates in the DC1 group were significantly lower than those in the DC2 group and the DC3 group(P<0.05). The D6 blastocyst formation rates in the DC1 group and the DC2 group were significantly lower than those in the normal cleavage group(P<0.05). The D6 blastocyst formation rates in the DC1 group were significantly lower than those in the DC2 group and the DC3 group(P<0.05). The D6 high-quality blastocyst formation rates in the DC1 group, the DC2 group and the DC3 group were significantly lower than those in the normal cleavage group(P<0.05). The D6 high-quality blastocyst formation rates in the DC1 group were significantly lower than those in the DC2 group and the DC3 group(P<0.05). Compared with those in the normal cleavage group, the biochemical pregnancy rate, clinical pregnancy rate, implantation rate and live birth rate in the DC1 group and the DC2/3 group were significantly decreased(P<0.05). Conclusion DC can reduce the embryonic development potential, and the earlier DC occurs, the greater the negative impact on the embryonic development. Time-lapse imaging technology is an effective method to monitor the occurrence of DC in the embryos and evaluate the embryonic development potential. |
| Key words: Time-lapse imaging Direct cleavage(DC) Embryonic development Pregnancy outcome |
