| 摘要: |
| ［摘要］ 目的 探讨白头翁皂苷B4（AB4）对雄激素性脱发（AGA）小鼠的治疗效果及作用机制。方法 选择C57BL/6雄性小鼠48只，将其随机分为对照组、模型组、AB4低剂量组、AB4中剂量组、AB4高剂量组及5%米诺地尔酊组，各8只。对照组小鼠正常喂养，不进行特殊干预。模型组及各干预组于小鼠颈背部外涂睾酮溶液［10 mg/（kg·d）］，建立AGA模型。完成AGA脱发模型构建后，模型组于小鼠颈背部外涂20%甘油溶液，2次/d，0.2 mL/次；AB4低剂量组、AB4中剂量组、AB4高剂量组分别于小鼠颈背部外涂5 mg/mL、10 mg/mL、20 mg/mL的AB4溶液，2次/d，0.2 mL/次；5%米诺地尔酊组于小鼠颈背部外涂对应药物，2次/d，0.1 mL/次。持续干预23 d，在干预期间于小鼠颈背部外涂睾酮溶液［5 mg/（kg·d）］，维持AGA模型。每周拍照观察记录小鼠毛发生长情况，并于最后一次干预结束后通过HE染色观察皮肤毛囊。通过酶联免疫吸附试验（ELISA）测定小鼠血清睾酮、雌二醇（E2）、Ⅱ型5-α还原酶（5AR2）水平并计算睾酮/E2比值；测定小鼠颈背部皮肤组织转化生长因子-β1（TGF-β1）、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）水平。结果 在结束23 d干预后，相比于模型组，AB4干预组小鼠皮肤毛囊数量显著增多（P<0.05），毛囊体积增大，且AB4中剂量组、AB4高剂量组的毛囊数量较5%米诺地尔酊组更多（P<0.05）。AB4干预组血清睾酮、5AR2以及睾酮/E2比值水平较模型组显著降低（P<0.05），E2水平显著升高（P<0.05）。AB4高剂量组血清E2水平较5%米诺地尔酊组更高，睾酮/E2比值较5%米诺地尔酊组更低，差异有统计学意义（P<0.05）。AB4干预组皮肤组织TGF-β1、IL-6水平均显著低于模型组和5%米诺地尔酊组（P<0.05）。结论 AB4可能通过调节血清激素平衡和抑制皮肤炎症反应发挥治疗AGA的作用。 |
| 关键词: 雄激素性脱发 白头翁皂苷B4 睾酮 雌二醇 Ⅱ型5-α还原酶 炎症指标 |
| DOI：10.3969/j.issn.1674-3806.2025.11.12 |
| 分类号:R 961 |
| 基金项目:广西科技计划项目（编号：桂科AA23026010） |
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| Study on therapeutic effect of anemoside B4 on mice with androgenetic alopecia and its mechanism of action |
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LIU Xiaoyan1,2, WANG Qinqin3, GAO Xiang1, XIE Xiaoyun1, CHEN Xiate1, YUAN Renyikun1, GAO Hongwei1, YANG Shilin1
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1.School of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China; 2.Department of Pharmacy, Chest Hospital of Guangxi Zhuang Autonomous Region, Liuzhou 545005, China; 3.School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330000, China
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| Abstract: |
| ［Abstract］ Objective To explore the therapeutic effect of anemoside B4(AB4) on androgenetic alopecia(AGA) in mice and its mechanism of action. Methods Forty-eight male C57BL/6 mice were selected and randomly divided into control group, model group, low-dose AB4 group, medium-dose AB4 group, high-dose AB4 group and 5% minoxidil tincture group, with 8 mice in each group. The mice in the control group were fed normally without special interventions. The model group and each intervention group were topically applied with testosterone solution［10 mg/(kg·d)］ on the skin on the neck and back of the mice to establish the AGA model. After construction of the AGA model, the model group was topically applied with 20% glycerol solution on the skin on the neck and back of the mice, twice daily, 0.2 mL per time, and the low-dose AB4 group, the medium-dose AB4 group and the high-dose AB4 group were topically applied with 5 mg/mL, 10 mg/mL and 20 mg/mL AB4 solution on the skin on the neck and back of the mice, respectively, twice daily, 0.2 mL per time, and the 5% minoxidil tincture group was topically applied with 5% minoxidil tincture on the skin on the neck and back of the mice, twice daily, 0.1 mL per time. The interventions lasted for 23 days, during which testosterone solution［5 mg/(kg·d)］ was topically applied on the skin on the neck and back of the mice to maintain the AGA model. The hair growth of the mice was observed and recorded by taking photos every week, and skin hair follicles were observed by using hematoxylin and eosin(HE) staining after the last intervention. The serum testosterone, estradiol(E2) and type Ⅱ 5-α reductase(5AR2) levels were detected by using enzyme-linked immunosorbent assay(ELISA) and the testosterone/E2 ratio was calculated. The levels of transforming growth factor-β1(TGF-β1), tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and interleukin-6(IL-6) in the skin tissues of the neck and back of the mice were detected. Results After 23 days of interventions, compared with the model group, the number of skin hair follicles in the AB4 intervention groups was significantly increased(P<0.05), with larger hair follicle volume. Moreover, the number of hair follicles in the medium-dose AB4 group and the high-dose AB4 group was larger than that in the 5% minoxidil tincture group(P<0.05). The levels of serum testosterone, 5AR2 and testosterone/E2 ratio in the AB4 intervention groups were significantly lower than those in the model group(P<0.05), while the levels of E2 in the AB4 intervention groups were significantly higher than those in the model group(P<0.05). The level of serum E2 in the high-dose AB4 group was higher than that in the 5% minoxidil tincture group, and the testosterone/E2 ratio in the high-dose AB4 group was lower than that in the 5% minoxidil tincture group, with statistically significant differences(P<0.05). The levels of TGF-β1 and IL-6 in skin tissues of the AB4 intervention groups were significantly lower than those in the model group and the 5% minoxidil tincture group(P<0.05). Conclusion AB4 may exert therapeutic effect on AGA by regulating serum hormone balance and inhibiting skin inflammatory response. |
| Key words: Androgenetic alopecia(AGA) Anemoside B4(AB4) Testosterone Estradiol(E2) Type Ⅱ 5-α reductase(5AR2) Inflammatory indicators |
