| 摘要: |
| ［摘要］ 目的 分析VITEK® 2 AST-YS08卡在非白念珠菌（NAC）药敏检测中的应用价值。方法 选择2017年1月至2021年12月从厦门市中医院和厦门大学附属第一医院住院患者正常无菌部位分离的NAC 159株，包括93株近平滑念珠菌群（近平滑念珠菌80株，拟平滑念珠菌8株，似平滑念珠菌4株，长孢洛德酵母1株），28株热带念珠菌，18株光滑念珠菌（包含1株尼瓦利亚念珠菌），18株季也蒙麦尔酵母（包含1株奥默柯达菌）以及2株库德里阿兹威毕赤酵母。分别采用Sensititre YeastOne法（SYO法）和VITEK® 2 AST-YS08卡平行检测NAC的最低抑菌浓度（MIC），依据美国临床实验室标准化协会（CLSI）发布的CLSI-M27S44标准进行结果判读。以SYO法结果为参考，分析VITEK® 2 AST-YS08卡结果的基本一致性（EA）、分类一致性（CA）、极严重错误（VME）、严重错误（ME）、微小错误（mE）。结果 以SYO法结果为参考，VITEK® 2 AST-YS08卡对NAC菌株在米卡芬净、氟康唑、伏立康唑、5-氟胞嘧啶、卡泊芬净的EA为91.82%～100.00%，CA为86.79%～95.12%，其中EA最高的是5-氟胞嘧啶（100.00%），其次为伏立康唑（99.29%）。VITEK® 2 AST-YS08卡对卡泊芬净的VME与拟平滑念珠菌菌株的MIC值与判读折点靠近有关。结论 VITEK® 2 AST-YS08卡操作简单，易于自动化。以SYO法结果为参考，其对NAC在氟胞嘧啶、米卡芬净、氟康唑、卡泊芬净和伏立康唑药敏结果的CA较高，具有较好临床应用价值。 |
| 关键词: 非白念珠菌 VITEK® 2 AST-YS08卡 药敏试验 基本一致性 分类一致性 |
| DOI：10.3969/j.issn.1674-3806.2025.12.11 |
| 分类号: |
| 基金项目:厦门市科技计划项目（编号：3502Z202373096） |
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| Analysis on application value of VITEK® 2 AST-YS08 card in drug sensitivity test of non-Candida albicans |
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LIU Weimin1, XU Heping2, ZHENG Yanqing2, MA Xiaobo2
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1.Department of Laboratory Medicine, Xiamen Hospital of Traditional Chinese Medicine, Xiamen 361003, China; 2.Department of Laboratory Medicine, the First Affiliated Hospital of Xiamen University, Xiamen Key Laboratory of Genetic Testing, Xiamen 361003, China
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| Abstract: |
| ［Abstract］ Objective To analyze the application value of VITEK® 2 AST-YS08 card in drug sensitivity test of non-Candida albicans(NAC). Methods A total of 159 strains of NAC isolated from the normal germfree sites of the inpatients in Xiamen Hospital of Traditional Chinese Medicine and the First Affiliated Hospital of Xiamen University from January 2017 to December 2021 were selected, including 93 strains of C. parapsilosis complex(80 strains of C. parapsilosis, 8 strains of C.orthopsilosis, 4 strains of C. metapsilosis and 1 strain of Lodderomyces elongisporus), 28 strains of C. tropicalis, 18 strains of C. glabrata(including 1 strain of C. nivariensis), 18 strains of Meyerozyma guilliermondii(including 1 strain of Kodamaea ohmeri) and 2 strains of Pichia kudriavzevii. The minimal inhibitory concentration(MIC) of NAC was tested in parallel using Sensititre YeastOne method(SYO method) and VITEK® 2 AST-YS08 card, respectively, and the results were interpreted according to the CLSI-M27S44 standard issued by the Clinical and Laboratory Standards Institute(CLSI). Taking the results of SYO method as the reference, the essential agreement(EA), category agreement(CA), very major error(VME), major error(ME) and minor error(mE) of the results were analyzed between VITEK® 2 AST-YS08 card and SYO method. Results Taking the results of SYO method as the reference, the EAs of the results in testing the sensitivity of NAC strains to micafungin, fluconazole, voriconazole, 5-flucytosine and caspofungin between VITEK® 2 AST-YS08 card and SYO method were 91.82% to 100.00%, and the CAs of the results were 86.79% to 95.12%. Among them, the EA of the results in 5-flucytosine sensitivity test was the highest(100.00%), followed by that in voriconazole sensitivity test(99.29%). The VME of the results in caspofungin sensitivity test by using VITEK® 2 AST-YS08 card was associated with the MIC values of the strains of C. orthopsilosis closing to the antimicrobial breakpoints for interpretation. Conclusion VITEK® 2 AST-YS08 card is easy to operate and convenient for automation. Taking the results of SYO method as the reference, the CAs of the results in testing the sensitivity of NAC strains to 5-flucytosine, micafungin, fluconazole, caspofungin and voriconazole between VITEK® 2 AST-YS08 card and SYO method are higher, and VITEK® 2 AST-YS08 card has better clinical application value. |
| Key words: Non-Candida albicans(NAC) VITEK® 2 AST-YS08 card Drug sensitivity test Essential agreement(EA) Category agreement(CA) |
