| 摘要: |
| ［摘要］ 目的 探究蒙药白益母草（PA）对人永生化子宫内膜基质细胞（St-T1b）增殖、迁移、侵袭及凋亡的影响，并揭示其对磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）信号通路的调控机制。方法 以St-T1b细胞为实验对象，设空白对照组（PA 0 mg/mL）、2 mg/mL PA组、4 mg/mL PA组、8 mg/mL PA组、740Y-P组、8 mg/mL PA联合740Y-P组。CCK-8检测细胞活力；Western blot检测细胞PI3K、AKT及磷酸化蛋白表达；JC-1检测细胞线粒体膜电位；calcein-AM/7-AAD双染色检测细胞凋亡；Transwell检测细胞迁移、侵袭；RT-qPCR检测B淋巴细胞瘤-2相关X蛋白（Bax）、B淋巴细胞瘤-2（Bcl-2）、基质金属蛋白酶2（MMP2）、基质金属蛋白酶9（MMP9）mRNA的相对表达量。经740Y-P回复验证佐证，PA作用机制与PI3K/AKT信号通路相关。结果 PA呈剂量依赖性抑制St-T1b细胞增殖（P<0.05）。与空白对照组相比，2 mg/mL PA组、4 mg/mL PA组、8 mg/mL PA组细胞线粒体膜电位、磷酸化磷脂酰肌醇3激酶（p-PI3K）及磷酸化蛋白激酶B（p-AKT）蛋白表达水平，Bcl-2、MMP2、MMP9的mRNA相对表达水平，以及细胞迁移与侵袭能力均显著下降（P<0.05）；细胞凋亡率、Bax mRNA相对表达水平显著升高（P<0.05）。与空白对照组比较，740Y-P组细胞线粒体膜电位、p-PI3K及p-AKT蛋白表达水平，以及Bcl-2、MMP2、MMP9的mRNA相对表达水平升高，细胞迁移、侵袭能力增强，细胞凋亡率、Bax mRNA相对表达水平降低，差异有统计学意义（P<0.05）。与8 mg/mL PA组相比，8 mg/mL PA联合740Y-P组线粒体膜电位、p-PI3K及p-AKT蛋白表达水平显著升高（P<0.05），细胞迁移、侵袭能力显著增强（P<0.05），Bcl-2、MMP2、MMP9的mRNA相对表达水平亦显著升高（P<0.05），细胞调亡率、Bax mRNA相对表达水平显著降低（P<0.05）。结论 PA可明显抑制St-T1b细胞增殖、迁移及侵袭，同时促进细胞凋亡，该生物学效应可能通过调控PI3K/AKT信号通路来实现。 |
| 关键词: 白益母草 子宫内膜异位症 PI3K/AKT信号通路 细胞迁移 细胞侵袭 细胞凋亡 |
| DOI：10.3969/j.issn.1674-3806.2026.06.06 |
| 分类号: |
| 基金项目:内蒙古医学科学院公立医院科研联合基金科技项目（编号：2024GLLH0371）；呼和浩特市卫生健康科技计划项目（编号：2024-呼卫科-009）；呼和浩特市“青城名医”工程医学创新人才项目（编号：呼青城名医-2025008） |
|
| Study on the effects of Mongolian herb Panzeria alaschanica Kupr on biological behaviors of endometriotic stromal cells and its association with the PI3K/AKT signaling pathway |
|
TIAN Manxue1, LI Xia2, DONG Zhiheng3, WEI Ni1, ZHAO Rongwei2,4
|
|
1.The First Clinical Medical School, Inner Mongolia Medical University, Hohhot 010059, China; 2.Department of Gynecology, Hohhot Maternal and Child Health Care Hospital, Hohhot 010020, China; 3.Department of Pharmacy, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, China; 4.Department of Gynecology, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, China
|
| Abstract: |
| ［Abstract］ Objective To explore the effects of Mongolian herb Panzeria alaschanica Kupr(PA) on proliferation, migration, invasion and apoptosis of immortalized human endometrial stromal cells(St-T1b), and to reveal its regulatory mechanism on the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT) signaling pathway. Methods St-T1b cells were used as experimental subjects, and a blank control group［PA(0 mg/mL)］, 2 mg/mL PA group, 4 mg/mL PA group, 8 mg/mL PA group, 740Y-P group and 8 mg/mL PA combined with 740Y-P group were established. CCK-8 assay was used to detect cell viability. Western blot was applied to detect the expressions of PI3K and AKT of cells and their phosphorylated forms. Mitochondrial membrane potential was detected by using JC-1 staining, and cell apoptosis was detected by using calcein-acetoxymethyl ester(calcein-AM)/7-aminoactinomycin D(7-AAD) double staining. Transwell assay was used to detect cell migration and invasion capacities. Reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR) was performed to detect the relative expressions of B-cell lymphoma-2-associated X protein(Bax), B-cell lymphoma-2(Bcl-2), matrix metalloproteinase 2(MMP2) and matrix metalloproteinase 9(MMP9) mRNAs. Rescue experiments were conducted via the intervention of 740Y-P and the results showed that the mechanism of action of PA was related to the PI3K/AKT signaling pathway. Results PA suppressed St-T1b cell proliferation in a dose-dependent manner(P<0.05). Compared with the blank control group, the PA-treated groups(the 2 mg/mL PA, 4 mg/mL PA and 8 mg/mL PA groups) showed significant decreases in mitochondrial membrane potential, protein expression levels of phosphorylated phosphatidylinositol 3-kinase(p-PI3K) and phosphorylated protein kinase B(p-AKT), and relative mRNA expression levels of Bcl-2, MMP2, and MMP9, as well as in cell migration and invasion capacities(P<0.05), and the PA-treated groups showed significant increases in the apoptotic rate and relative Bax mRNA expression level(P<0.05). Compared with the blank control group, the 740Y-P group upregulated the mitochondrial membrane potential of the cells, the protein expression levels of p-PI3K and p-AKT, the relative mRNA expression levels of Bcl-2, MMP2 and MMP9, enhanced the cell migration and invasion capacities, as well as downregulated the apoptotic rate and relative Bax mRNA expression level, with statistically significant differences(P<0.05). Compared with the 8 mg/mL PA group, the 8 mg/mL PA combined with 740Y-P group significantly elevated the mitochondrial membrane potential and the protein expression levels of p-PI3K and p-AKT(P<0.05), significantly enhanced the cell migration and invasion capacities(P<0.05), and significantly elevated the relative mRNA expression levels of Bcl-2, MMP2 and MMP9(P<0.05), and significantly downregulated the apoptotic rate and relative Bax mRNA expression level(P<0.05). Conclusion PA can significantly suppress the proliferation, migration and invasion of St-T1b cells, and induce cell apoptosis. These biological effects may be achieved by regulating the PI3K/AKT signaling pathway. |
| Key words: Panzeria alaschanica Kupr(PA) Endometriosis PI3K/AKT signaling pathway Cell migration Cell invasion Apoptosis |
