| 摘要: |
| ［摘要］ 目的 利用小干扰RNA（small interfering RNA, siRNA）抑制人舌癌Tca8113细胞血管内皮生长因子(vascular endothelial growth factor，VEGF)表达，观察其对培养细胞和移植瘤基质金属蛋白酶-9(matrix metalloproteinase-9，MMP-9)蛋白表达的影响。方法 以稳定转染携带VEGF-siRNA真核表达载体的人舌癌Tca8113细胞（VEGF-siRNA1、VEGF-siRNA2）为实验组，以转染空载体人舌癌Tca8113细胞及未转染的人舌癌Tca8113细胞为实验对照组和空白对照组，将各组细胞分别接种于裸鼠皮下，用免疫细胞/组织化学法分别测定各组培养细胞和移植瘤VEGF及MMP-9的蛋白表达。结果 在培养细胞和移植瘤中，与实验对照组和空白对照组相比，VEGF-siRNA1组和VEGF-siRNA2组VEGF染色平均灰度值高，差异有统计学意义(P<0.05)；各组培养细胞中均未见MMP-9表达；各组移植瘤MMP-9染色平均灰度值之间的差异无统计学意义（P>0.05）。 结论 以siRNA干扰沉默人舌癌Tca8113细胞VEGF基因，可明显抑制细胞及移植瘤内VEGF蛋白表达，但是对MMP-9蛋白的表达无明显影响。 |
| 关键词: 小干扰RNA 舌癌 基质金属蛋白酶 血管内皮生长因子 |
| DOI：10.3969/j.issn.1674-3806.2009.06.01 |
| 分类号:Q 753 |
| 基金项目:国家自然科学基金资助项目(No.30360111) |
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| The expression of MMP-9 after the expression of VEGF suppressed by siRNA in human tongue squamous cell carcinoma |
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HAO Jie, YU Da-hai, LI Jing, et al.
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Department of Oral and Maxillofacial Surgery, College of Stomatology, Guangxi Medicial University, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To investigate the expression of matrix metalloproteinase-9 (MMP-9) in human tongue squamous cell carcinoma（Tca8113）in vitro and in vivo, after the expression of vascular endothelial growth factor（VEGF）suppressed by vector-based small interfering RNA(siRNA).Methods The eukaryotic expression vector taken as an experimental control and two siRNA targeting VEGF constructed in the vector (VEGF-siRNA1, VEGF-siRNA2) were transfected into human tongue squamous cell carcinoma cell lines(Tca8113). The non-transfected Tca8113 cells were used as negative control. The transfected cells and non-transfected cells were injected subcutaneously on the back of the twenty nude-mice which were divided into four groups randomly (n=5/group), respectively. The expression of VEGF and MMP-9 in the cultured cells and the xenograft tumors were detected by immunocytochemistry and immunohistochemistry.Results Compared with the experimental and negative controls, the VEGF expressions were decreased (P<0.05) in two experimental groups of the cultured cells and xenograft tumors. There was no obvious MMP-9 staining in four groups of cultured cells. There were no significant differences of the MMP-9 expression among four groups of xenograft tumor (P>0.05).Conclusion VEGF-siRNA can down-regulate the expression of VEGF in the human tongue squamous cell carcinoma in vitro and in vivo. However, it may not influence on the expression of MMP-9 efficiently. |
| Key words: Small interfering RNA(siRNA) Tongue squamous cell carcinoma Matrix metalloproteinases(MMPs) Vascular endothelial growth factor（VEGF） |
