| 摘要: |
| ［摘要］ 目的 观察人舌癌裸鼠皮下移植瘤模型中肿瘤新生血管内皮细胞的来源。方法 通过皮下移植人舌癌Tca8113-Ml细胞建立人舌癌裸鼠移植肿瘤模型, 并于肿瘤生长至直径1cm时切除肿瘤, 对移植瘤进行病理组织学观察, 利用免疫组织化学方法检测移植瘤内新生血管内皮细胞的来源，抗体为鼠抗人单克隆抗体CD34和大鼠抗小鼠单克隆抗体CD34；并进行微血管密度（microvessel density，MVD）计数及血管内皮生长因子（vascular endothelial growth factor, VEGF）表达检测。结果 右腋窝皮下接种人舌癌Tca8113-Ml细胞2周后，6只裸鼠的肿瘤直径都达到1 cm 以上，切片HE染色可见肿瘤组织病理形态为鳞状上皮细胞癌，MVD平均值为10.72±2.12，其中肿瘤新生血管内皮细胞大鼠抗小鼠CD34免疫组化结果是阳性，而鼠抗人CD34阴性。VEGF在6例移植瘤标本中均阳性表达, 平均阳性率为（67±5.6）%。结论 人舌癌移植瘤模型中血管内皮细胞主要来源于宿主裸鼠，并且可能与肿瘤细胞分泌的VFGE诱导有关。 |
| 关键词: 舌癌 Tca8113-Ml细胞 血管生成 血管内皮细胞 |
| DOI：10.3969/j.issn.1674-3806.2009.06.02 |
| 分类号:Q 343 |
| 基金项目:国家自然基金资助项目（No. 30860326），广西自然科学基金资助项目（No.桂科自 0640202），广西教育厅资助项目［桂教科研(2005)47号］ |
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| The origin of vascular endothelial cell in nude mice bearing human tongue carcinoma |
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ZHU Ming-yi, KUANG Xiao-cong, HUANG Ying-hua, et al.
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Department of Pathophysiology, Guangxi Medical University, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the origin of vascular endothelial cells in human tongue carcinoma xenograft model by nude mice.Methods The model of human tongue carcinoma xenograft was established by transplanting Tca8113-Ml subcutaneously to nude mice, to identify the germinal origin of neovascularization and assay the microangiogenesis, the neoplasms(>1cm in diameter) induced by Tca8113-Ml were removed and dealt with histopathologically through immunohistochemical staining of VEGF and CD34(including rat -anti-human and rat-anti-mouse CD34).Results HE staining showed squamous cell carcinoma; positive expression was observed in rat-anti-mouse CD34 staining and MVD averaged 10.72±2.12, meanwhile no visible alteration of rat-anti-human CD34 staining;moreover, VEGF staining presented a 100% positive ration in six transplanted tumor and the numbers of positive cells averaged (67±5.6)% in every slice.Conclusion The germinal origin of vascular endothelial cells in Tca8113-Ml xenograft model derived from host nude mice, and may be induced by VEGF which is secreted by tumor cells. |
| Key words: Tongue carcinoma Tca8113-Ml cell Angiogenesis Vascular endothelial cell |
