| 摘要: |
| ［摘要］ 目的 探讨合成芥酸乙醇酰胺对人肝癌细胞BEL-7404的抑制作用。方法 (1)运用微型有机合成技术，以芥酸、甲醇和乙醇胺为原料、金属钠为催化剂合成芥酸乙醇酰胺，采用硅胶柱层析分离纯化，红外光谱和核磁共振谱确证结构；(2)用芥酸乙醇酰胺作用于BEL-7404，用倒置显微镜镜下观察其对BEL-7404细胞的生长状况的影响，MTT法观察其抑制作用。结果 合成出了芥酸乙醇酰胺并确证了其结构；芥酸乙醇酰胺浓度在50.0 μg/ml以上时，能很明显的抑制BEL-7404的增殖，抑制率均高于40%，IC50为101.77588 μg/ml。结论 采用微型合成法能够快速、方便地合成出芥酸乙醇酰胺；芥酸乙醇酰胺对BEL-7404细胞的增殖有明显的抑制作用。 |
| 关键词: 芥酸 芥酸乙醇酰胺 BEL-7404 抗肿瘤作用 |
| DOI：10.3969/j.issn.1674-3806.2010.03.03 |
| 分类号:R 735.7 |
| 基金项目:国家自然科学基金资助项目（项目编号：30560186）；教育部“春晖计划”项目（项目编号：教外司［2003］593）；广西壮族自治区科技厅科学基金项目（编号：桂科自0728128）；2007年广西研究生教育创新计划项目 |
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| Microscale synthesis of N-alcohol erucamide and its inhibiting effect on BEL-7404 in vitro |
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GAO Ming-xing, QIN Qing, MO De-huan,et al.
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Guangxi Medical University, Nanning 530021,China
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| Abstract: |
| ［Abstract］ Objective To study the synthesis of the N-alcohol erucamide and its activity of anti-tumor in vitro.Methods (1)Through the micro-organic synthesis technology, erucic acid and methanol were coupled with ethanolamine as raw matierals, sodium as catalyst to synthesize the N-alcohol erucamide, isolation and purification achieved by silical gel column chromatography, and Its structure was confirmed by infrared spectra and nuclear magnetic resonance spectrum; (2)BEL-7404 was cultured in media added to the N-ethanol erucamide.The cells′s growth was observed in inverted microscope. The effect of inhibiting cancer cells was detected with MTT.Results The N-alcohol erucamide was synthesized successfully；Its strong inhibiting effect was recorded beyond 50.0 μg/ml.The inhibiting rates were all beyond 40%, the inhibitory concentration 50% (IC50) was 101.77588 μg/ml.Conclusion Through the microscale organic synthesis, N-alcohol erucamide can be synthesized quickly and conveniently. N-alcohol erucamide has obvious inhibiting effcet on BEL-7404 cells in vitro. |
| Key words: Erucic acid N-alcohol erucamide BEL-7404 Anti-tumor |
