| 摘要: |
| ［摘要］ 目的 探讨联合测定胸水中TOLL样受体2(TLR2)和γ-干扰素（IFN-γ）水平对结核性胸腔积液的诊断价值。方法 收集确诊结核性胸腔积液、恶性胸腔积液和细菌性胸腔积液患者的胸水和血清，应用酶联免疫吸附试剂盒(ELISA)测定TLR2和IFN-γ的浓度，并对数据进行分析。结果 （1）结核组患者胸水TLR2（22.19±2.12）ng/L、IFN-γ(2 287.20±408.34)ng/L均比其相应血清水平高，差异有统计学意义（P<0.05）。结核组患者胸水的TLR2和IFN-γ水平均高于恶性组和细菌组，差异有统计学意义（P<0.05）。（2）TLR2诊断结核性胸腔积液的工作特征曲线(ROC)下面积（ACU）为0.696(95%CI:0.501～0.849),灵敏度为66.67%,特异度为80%。同样，IFN-γ的ACU为0.945(95%CI:0.875～1.014),灵敏度为87.5%,特异度为97.9%。结论 联合测定TLR2和IFN-γ指标有助于结核性胸腔积液的诊断。 |
| 关键词: 结核性胸腔积液 TOLL样受体2 γ-干扰素 |
| DOI：10.3969/j.issn.1674-3806.2013.08.02 |
| 分类号:R 56 |
| 基金项目:广西自然科学基金资助项目（编号：桂科自0728081）；广西卫生厅科研课题（编号：Z2013394） |
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| Value of combined detection of Toll-like receptor 2 and interferon-γ in the diagnosis of tuberculous pleural effusion |
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HUANG Lu-ying, WU Yan-bin, FENG Ting-mei, et al.
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Department of Respiratory Diseases, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the value of the combined detection of Toll-like receptor-2(TLR2) and interferon-γ(IFN-γ) in pleural effusion in the diagnosis of tuberculous pleural effusion.Methods The concentrations of soluble TLR2 and IFN-γ in pleural effusion and serum were determined by enzyme linked immunosorbent assay(ELISA) in patients with tuberculous, malignant and bacterial pleural effusion.Results （1）The concentrations of sTLR2［22.19±2.12）ng/L］ and IFN-γ［(2 287.20±408.34)ng/L］ in pleural effusions were significantly higher than those in serum of the same patients. The levels of sTLR2 and IFN-γ in tuberculous pleural effusion was significantly higher than those in malignant and in bacterial pleural effusion.（2）The ROC analysis for TLR2 and IFN-γ were done. The levels of TLR2 generated an area under the receive operating characteristic(ROC) curve of 0.696［95% confidence interval (CI):0.501～0.849］, at a sensitivity of 66.67%, a specificity of 80%. As well as IFN-γ generated an area under the ROC curve of 0.945(95%CI:0.875～1.014), at a sensitivity of 87.5%, a specificity of 97.9 %.Conclusion The combined decection of TLR2 and IFN-γ in pleural effusion may be helpful in the differential diagnosis of tuberculous pleural effusion and the other pleural effusion, which provides an important information for clinical diagnosis and treatment. |
| Key words: Tuberculous pleural effusion Toll-like receptor-2 Interferon-γ |
