| 摘要: |
| ［摘要］ 目的 比较全自动尿液分析仪（干化学法）、全自动尿沉渣分析仪（尿沉渣法）与人工镜检法检测尿中白细胞的结果。方法 分别采用干化学法、尿沉渣法和人工镜检法对采集的845份尿标本进行白细胞测定，并对结果进行比较分析。结果 以人工显微镜检测结果作为金标准，845份受检尿液中，干化学法分析尿液白细胞阳性符合率为89.69%，阴性符合率为66.82%，假阳性率为33.12%，假阴性率为10.31%，阳性似然比为2.71，阴性似然比为0.15，总符合率为72.07%，两者结果比较差异有统计学意义（P<0.01）；尿沉渣法分析尿液白细胞阳性符合率为95.87%，阴性符合率为83.56%，假阳性率为16.44%，假阴性率为4.13%，阳性似然比为5.80，阴性似然比为0.05，总符合率为86.39%，两者结果比较差异有统计学意义（P<0.01）；干化学法与尿沉渣法比较阳性符合率为89.41%，阴性符合率为76.81%，假阳性率为23.19%，假阴性率为10.59%，总符合率为81.18%，阳性似然比为3.85，阴性似然比为0.13，两者结果比较差异有统计学意义（P<0.01）。结论 尿液中有形成分复杂，全自动仪器法不能完全取代显微镜镜检，只是大批量标本分析时的一种过筛手段，对检测结果阳性者仍需用显微镜复检,以提高报告结果的准确性。三种方法联合应用，可以提高检测结果的准确度，具有较高的临床应用价值。 |
| 关键词: 仪器法 人工镜检法 白细胞 |
| DOI：10.3969/j.issn.1674-3806.2013.11.10 |
| 分类号:R 446.12 |
| 基金项目: |
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| Comparative analysis of white blood cells in urine determined by automatic instruments and artificial microscopy |
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LI Hang， MA Chun-yan， LI Xiang-lei，et al.
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Department of Clinical Laboratory， Center Hospital of Kaifeng, Henan 475000, China
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| Abstract: |
| ［Abstract］ Objective To compare the result of white blood cells in the urine by the automated urine analyzer(dry chemical method), automatic urinary sediment analyzer and artificial microscopy.Methods Three different methods(dry chemistry method ， urinary sediment method and artificial microscopy）were used for determination of white cells in 845 urine samples,and the results were compared.Results Using the microscope detection results as the gold standard in 845 urine samples, the detection of dry chemical method for urine leukocyte showed the positive coincidence rate was 89.69%,the negative coincidence rate was 66.82%, the false positive rate was 33.12%, the false negative rate was 10.31%, the positive likelihood ratio was 2.71, the negative likelihood ratio was 0.15, the total coincidence rate was 72.07%, comparing the results of the two methods, the difference was statistically significant(P<0.01). The detection of urinary sediment method for urine leukocyte showed the positive coincidence rate was 95.87%, the negative coincidence rate 83.56%, the false positive rate is 16.44%, the false negative rate was 4.13%, the positive likelihood ratio was 5.80, the negative likelihood ratio was 0.05, the total coincidence rate 86.39%, comparing the results of the two methods, the difference was statistically significant(P<0.01).The comparison between the dry chemical method and urinary sediment method showed the positive coincidence rate was 89.41%, the negative coincidence rate 76.81%, the false positive rate is 23.19%, the false negative rate was 10.59%, the total coincidence rate was 81.18%, the positive likelihood ratio was 3.85,the negative likelihood ratio was 0.13, there was significant statistically differences between two methods(P<0.01).Conclusion Urine visible components are complex, full automatic instrument method can not completely replace the microscopy and can only be used as a sieving mothod in analysis of large quantities of samples, so its positive test results still need to use the microscopy to improve accuracy in reporting results. The combination of the three kinds of methods can improve the accuracy of the detection results and have great value in clinical application. |
| Key words: Instrument method Artificial microscopy White blood cell |
