| 摘要: |
| ［摘要］ 目的 探讨广西巴马小型猪浅低温心脏不停跳体外循环（CPB）模型的建立。方法 选用32～40 kg健康的广西巴马小型猪13只，10只用于实验，3只用于取血预充。全麻下胸正中切口，心包外法建立体外循环。阻断上、下腔静脉，不阻断主动脉，不灌注心脏停跳液，维持心脏窦性节律空跳。CPB期间保持心率（HR）50～80次/min，鼻咽温32～34 ℃，灌注流量80～100 ml·kg-1·h-1，平均动脉压（MAP）60～80 mmHg，红细胞压积（HCT）20%～25%。结果 1只气管插管时误入食道缺氧死亡；1只在分离下腔静脉时破裂出血，经修补和血液回收回输后，生命体征平稳；1只开始转流时出现短暂室颤，未予处理自行转为窦性心率；2只HR>80次/min，排除麻醉原因后，予机器注入艾司洛尔0.5 mg/kg。CPB期间尿量（800±105）ml，2只有一过性浅红血尿。结论 广西巴马小型猪CPB，可以为CPB下不停跳心内手术开展研究提供合适的动物模型。 |
| 关键词: 浅低温 不停跳 体外循环 广西巴马小型猪 |
| DOI：10.3969/j.issn.1674-3806.2014.02.03 |
| 分类号:R 654.1 |
| 基金项目:国家自然科学基金资助项目（编号：81060027）；广西青年科学基金资助项目（编号：桂科青0991063） |
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| Establishment of mild hypothermic cardiopulmonary bypass on beating heart for Guangxi bama miniature pig |
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HU Yan-yan,LIN Hui,HUANG Ai-lan,et al.
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Department of Anesthesiology, Department of Cardiothoracic surgery,the People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China
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| Abstract: |
| ［Abstract］ Objective To investigate of the establishment of mild hypothermic cardiopulmonary bypass(CPB) on beating heart for Guangxi bama miniature pig.Methods Thirteen bama pigs weighted 32～40 kg were chosen,3 pigs were used for sampling blood,another 10 pigs were used for cardiopulmonary bypass.Pigs underwent chest incision under general anesthesia.The superior vena cava and the inferior vena cava were circumferentially mobilized and encircled with ties outside the pericadium to establish CPB,blocking superior and inferior vena cava cannulas without cross-clamping the aorta.The heart rate was maintained 50 to 80 bpm,the nasopharyngeal temperature was kept at 32～34 ℃,flow volumes were 80～100 ml·kg-1·h-1,mean arterial blood pressure were 60～80 mmHg,HCT were 20%～25% during CPB procedure.Results One pig was died of hypoxia induced intubation to esophagus. One pig loss excessive blood when disassociated the inferior vena cave. One pig appeared transient ventricular fibrillation for 4s when CPB initiated.Esmolol was used when HR over 80bpm to induce adequate bradycardia effect.In two cases mild hemoglobinuria was experienced.Conclusion Establishment of mild hypothermic cardiopulmonary bypass on beating heart for Guangxi bama miniature pig was a ideal model for cardiac surgery on beating heart under CPB. |
| Key words: Mild hypothermia Beating heart CPB Guangxi bama miniature pig |
