| 摘要: |
| ［摘要］ 目的 检测肺结核患者支气管肺泡灌洗液中IL-10水平，探索IL-10对肺结核的诊断意义。方法 采用ELISA双抗体夹心法测定50例肺结核患者及48名健康体检者外周血IL-10水平，同时测定肺结核患者抗结核治疗前后支气管肺泡灌洗液中IL-10水平，观察其差异有无统计学意义。结果 肺结核患者外周血中IL-10水平明显高于健康对照组，肺结核患者经抗结核治疗后外周血和支气管肺泡灌洗液中IL-10水平均较治疗前下降。结论 在结核病的发生过程中IL-10发挥了重要的免疫抑制作用，它对临床上肺结核的诊断有一定辅助意义。 |
| 关键词: 肺结核 支气管肺泡灌洗液 白细胞介素-10 |
| DOI：10.3969/j.issn.1674-3806.2015.01.04 |
| 分类号:R 521 |
| 基金项目:南京市医学科技发展课题(编号：YKK12126) |
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| The value of detecting the level of IL-10 in bronchoalveolar lavage fluid for tuberculosis diagnosis |
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WANG Juan, GE Hai-Bo, SUN Si-qing, et al.
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Department of Tuberculosis, Nanjing Chest Hospital，Jiangsu 210009，China
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| Abstract: |
| ［Abstract］ Objective To detect the the level of IL-10 in bronchoalveolar lavage fluid and explore the diagnostic value of IL-10 in tuberculosis.Methods The double-antibody sandwich ELISA methods were used to detect the expression of IL-10 in serum of 50 patients with pulmonary tuberculosis and 48 healthy people. The expression of IL-10 in bronchoalveolar lavage fluid of 50 patients with pulmonary tuberculosis was detected before anti- tuberculosis therapy and after 4 weeks′ therapy and compared their statistical differences.Results The level of IL-10 in peripheral blood of patients with pulmonary tuberculosis was significantly higher than that of the healthy people. The level of IL-10 in both peripheral blood and in bronchoalveolar lavage fluid of patients with pulmonary tuberculosis decreased after anti-tuberculosis therapy than those before therapy.Conclusion IL-10 plays an important role on immunosuppression in the the process of development of tuberculosis and it may assist in diagnosing tuberculosis in the clinical work. |
| Key words: Pulmonary tuberculosis Bronchoalveolar lavage fluid IL-10 |
