| 摘要: |
| ［摘要］ 目的 检测大鼠突出椎间盘组织及血液中基质金属蛋白酶3（MMP-3）含量的变化，探讨MMP-3保守治疗椎间盘突出症的可行性。方法 随机将40只3月龄SD大鼠，分为正常对照组、阴性对照组、实验组1及实验组2，每组10只。造模45 d后，用ELISA法检测椎间盘突出组织及血液中MMP-3含量。结果 （1）与正常对照组相比，实验组1、2的椎间盘组织及血液MMP-3浓度上升，差异有统计学意义(P<0.05)。（2）实验组1椎间盘组织及血液MMP-3浓度较实验组2明显升高，差异有统计学意义（P<0.05）。（3）实验组1、2椎间盘组织中MMP-3浓度的变化与血液中MMP-3相关系数分别为0.835和0.924，均P<0.05。结论 椎间盘突出再吸收过程中，血液中MMP-3含量上升，且局部椎间盘组织与血液中MMP-3的含量高度正相关，临床上运用MMP-3局部注射非手术方法治疗椎间盘突出症的设想可能行不通。 |
| 关键词: 椎间盘突出 再吸收 基质金属蛋白酶 酶联免疫吸附试验 |
| DOI：10.3969/j.issn.1674-3806.2015.03.04 |
| 分类号:R 318 |
| 基金项目:广西医疗卫生适宜技术研究与开发项目(编号：S201407-01)；桂林市科学研究与技术开发计划项目(编号：20140120-1-14) |
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| Expression and significance of MMP-3 in prominent intervertebral disc tissue and blood in rat model of intervertebral disc herniation |
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QIN Jin-ding, JIANG Yao-chuan, XIAO Rong-chi
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Department of Orthopaedics, the Affiliated Hospital of Guilin Medical University, Guangxi 541001, China
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| Abstract: |
| ［Abstract］ Objective To detect the change of MMP-3 in the prominent intervertebral disc tissue and blood in rats of intervertebral disc herniation to explore the feasibility of conservative treatment for intervertebral disc herniation with MMP-3.Methods Forty SD rats(only 3 months of age) were randomly divided into normal control group, negative control group, experimental group 1 and experimental group 2, 10 in each group. The MMP-3 content in disc herniation organization and the blood were detected with enzyme-linked immunosorbent assay method after 45 days of experiment.Results (1)The expression of MMP-3 was significantly higher in the experimental group 1 and group 2 compared with that of normal control group(P<0. 05); (2)The express of MMP-3 was significantly higher in group 1 compared with that of group 2(P<0. 05); (3)the concentration of MMP-3 between intervertebral disc tissue and blood in group 1 and group 2, had a linearly relating, and the correlation coefficients were 0.835, and 0.924(P<0. 05).Conclusion The content of MMP-3 in the blood rises in the process of intervertebral disc herniation resorption, and content of MMP-3 in local intervertebral disc tissue is highly correlated with the level of that in the blood, idea of nonsurgical treatment by local injections with MMP-3 to cure intervertebral disc herniation may not come true in the clinical use. |
| Key words: Intervertebral disc erniation Resorption MMP-3 Enzyme-linked immunosorbent assay |
