| 摘要: |
| ［摘要］ 目的 探讨甲强龙对实验性变态反应性脑脊髓炎（EAE）小鼠的肿瘤坏死因子α（TNF-α）和白介素17（IL-17）的影响。方法 将30只雌性C57BL/6J小鼠随机分为甲强龙组、EAE组和正常组，每组10只。EAE模型通过以髓鞘少突胶质细胞糖蛋白多肽35-55（MOG35-55）为抗原诱导。采用蛋白质印迹法（Western Blot）检测小鼠大脑的TNF-α和IL-17表达水平。结果 甲强龙组的临床症状较EAE组改善（P<0.05），大脑的TNF-α和IL-17表达水平明显低于EAE组（P<0.05）。结论 甲强龙能改善EAE小鼠的症状，其作用机制之一是抑制促炎性因子TNF-α和IL-17的表达。 |
| 关键词: 甲强龙 实验性变态反应性脑脊髓炎 多发性硬化 肿瘤坏死因子α 白介素17 |
| DOI：10.3969/j.issn.1674-3806.2015.08.02 |
| 分类号:R 742 |
| 基金项目:广西医疗卫生重点科研课题（编号：重2010028）;广西自然科学基金资助项目（编号：2011GXNSFA018185）;国家自然科学基金资助项目（编号：81460192） |
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| Effect of methylprednisolone on the levels of TNF-α and IL-17 in mice with experimental autoimmune encephalomyelitis |
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LI Xiao-feng, LI Lv-li, WEI Jun-jie, et al.
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Department of Neurology, the People′s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To explore the effects of methylprednisolone on the levels of TNF-α and IL-17 in the central nervous system of mice with experimental autoimmune encephalomyelitis(EAE).Methods 30 female C57BL/6J mice were randomly divided into 3 groups: methylprednisolone-treated group, EAE group and normal group. The EAE model was induced by immunizing mice with myelin oligodendrocyte glycoprotein peptides(MOG35-55). The brain tissues were collected and their expressions of TNF-α and IL-17 were detected using Western Blot.Results The neurological deficits of the methylprednisolone-treated mice were lighter than those of the EAE mice(P<0.05), while the methylprednisolone-treated mice displayed the lower expressions of TNF-α and IL-17 than the EAE mice did(P<0.05).Conclusion Methylprednisolone can protect against EAE in mice by the inhibition of TNF-α and IL-17. |
| Key words: Methylprednisolone Experimental autoimmune encephalomyelitis(EAE) Multiple sclerosis TNF-α IL-17 |
