| 摘要: |
| ［摘要］ 目的 通过构建慢性肝损伤性胆石症模型，利用大黄灵仙颗粒药物干预，探究EGR-1在防治肝损伤胆石症过程中的表达变化。方法 利用腹腔注射CCl4-橄榄油溶液构建兔慢性肝损伤模型，同时予高热量、高胆固醇致石饲料，建立兔慢性肝损伤胆石症模型。模型构建成功后，治疗组采用大黄灵仙颗粒0.4 g/（kg·d）进行干预，其余各组大白兔均正常饮食。用药12周后，麻醉后取肝脏组织，分别通过RT-PCR、免疫组化等方法进行检测。结果 （1）免疫组化结果：与各模型组相比，喂食大黄灵仙颗粒剂的各治疗组大白兔EGR-1的阳性表达明显减少，表现为肝脏汇管区及肝窦的肝细胞胞浆上棕褐色颗粒沉积表达明显减少，说明用药后可减少EGR-1蛋白的阳性表达使受损肝功能得到改善。（2）RT-PCR结果：各治疗组的EGR-1 mRNA的表达量均有不同程度降低（P均<0.05），说明大黄灵仙颗粒可通过减少EGR-1 mRNA表达量有效减轻肝损伤。结论 大黄灵仙颗粒可以有效预防结石的形成，减少慢性肝损伤性胆石症动物模型EGR-1的mRNA与蛋白的表达量，具有减轻肝损伤及改善肝功能的作用。 |
| 关键词: 慢性肝损伤 胆石症 EGR-1 基因 蛋白 |
| DOI：10.3969/j.issn.1674-3806.2016.11.01 |
| 分类号:R 575.6+2 |
| 基金项目:国家自然科学基金资助项目（编号：81470198）；广西科学研究与技术开发计划项目（编号：桂科攻14123006-3） |
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| A study of Dahuanglingxian granule on treating liver fibrosis cholelithiasis in rabbit model by intervening and regulating the expression of EGR-1 |
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WANG Bing, TANG Qian-li, WANG Yu, et al.
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Department of Emergency, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China
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| Abstract: |
| ［Abstract］ Objective To explore the expression of EGR-1 in the prevention and treatment of liver fibrosis cholelithiasis in rabbit model by using Dahuanglingxian granule.Methods The rabbit model of chronic liver fibrosis was established by intra-peritoneal injection of CCl4- olive oil, and given high-calorie diet and high-cholesterol diet; After the rabbit model was successfully established, the treatment group was treated with Dahuanglingxian granules 0.4 g/（kg·d）, and the other groups were on normal diet. The liver tissues of all the rabbits were tested by PCR and immunochemistry staining 12 weeks after the treatment.Results （1）Immunochemistry staining: Compared with that in the model group, the positive expression of EGR-1 reduced obviously, and the less brown particles were observed in the cytoplasm and membrane of live cells in sinusoids and hepatic duct area in the treatment group, suggesting the positive effects of Dahuanglingxian granules on liver damage.（2）Results of RT-PCR: The mRNA levels of EGR-1 in the groups treated with Dahuanglingxian granules were decreased(P<0.05), indicating that this drug could effectively reduce the live damage by reducing the mRNA levels of EGR-1.Conclusion Dahuanglingxian granules can effectively prevent the formation of cholelithiasis, reduce the mRNA and protein expression of EGR-1 in rabbit model of liver fibrosis cholelithiasis, and decrease the liver damage and improve the liver function. |
| Key words: Chronic liver injury Cholelithiasis EGR-1 Gene Protein |
