| 摘要: |
| ［摘要］ 目的 评价白藜芦醇(resveratrol,Res)预处理对大鼠肠缺血再灌注继发肺损伤时NF-κB活性的影响。方法 选健康清洁级雌性SD大鼠24只，体重200～220 g，采用随机数字表法分为3组（n=8）：假手术组（S组）、肠缺血再灌注组（M组）和白藜芦醇预处理组（R组）。采用夹闭肠系膜上动脉75 min后再灌注4 h的方法制备肠缺血再灌注继发性肺损伤（IIR-ALI）模型。于模型制备前5 d，R组每天腹腔注射Res 15 mg/kg（采用生理盐水稀释），S组和M组腹腔注射等容量生理盐水。于再灌注4 h时放血处死大鼠，收集肺组织和支气管肺泡灌洗液标本，测肺湿干重比值（W/D）、支气管肺泡灌洗液蛋白浓度、支气管肺泡灌洗液中IL-1β和TNF-α含量以及光镜下行肺损伤评分（Hofbauer评分)；采用免疫印迹检测肺组织核蛋白NF-κB p65的表达。结果 与S组比较，M组和R组大鼠肺组织损伤严重，肺组织损伤评分、肺W/D值、支气管肺泡灌洗液蛋白浓度、支气管肺泡灌洗液蛋白浓度IL-1β和TNF-α含量以及肺组织核蛋白NF-κB p65的表达增加（P<0.05）；与M组比较，R组大鼠肺组织损伤减轻，肺组织损伤评分、肺W/D值、支气管肺泡灌洗液蛋白浓度、支气管肺泡灌洗液蛋白浓度IL-1β和TNF-α含量以及肺组织核蛋白NF-κB p65表达下降（P<0.05）。结论 白藜芦醇预处理可减轻大鼠肠缺血再灌注继发性肺损伤，机制可能与其抑制肺组织NF-κB活性有关。 |
| 关键词: 白藜芦醇 再灌注损伤 急性肺损伤 核因子κB 细胞因子 |
| DOI：10.3969/j.issn.1674-3806.2017.12.04 |
| 分类号:R 9 |
| 基金项目:广东省深圳市科技计划项目（编号：JCYJ20150402090413031） |
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| Resveratrol preconditioning attenuates acute lung injury induced by intestinal ischemia-reperfusion in rats by inhibiting NF-kappaB activity |
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YANG Jian-yu, HUANG Xiao-lei, LI Bao-zhen, et al.
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Department of Anesthesiology, Shenzhen Maternity and Child Healthcare Hospital Affiliated to Southern Medical University, Guangdong 518028, China
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| Abstract: |
| ［Abstract］ Objective To evaluate the effect of resveratrol preconditioning on nuclear factor of kappa B(NF-kappaB) activity during acute lung injury induced by intestinal ischemia-reperfusion(IIR) in rats.Methods Twenty-four healthy female SD rats were randomly divided into three groups: sham-operated group(S group, n=8), intestinal ischemia-reperfusion mode group(M group, n=8), resveratrol pretreatment group(R group, n=8). Acute lung injury induced by intestinal ischemia-reperfusion(IIR-ALI) was conducted by clamping the superior mesenteric artery(SMA) in rats for 75 minutes followed by 4 hours′ reperfusion. In the R group, rats were treated with resveratrol(15 mg/kg, intraperitoneally) for 5 days before IIR. In the other groups, rats were treated with the same volume of NS for 5 days before operation. All rats were sacrificed after 4 hours′ reperfusion of SMA. The lung tissues were obtained for calculation of wet/dry weight ratio(W/D) and pathological investigation(Hofbauer′s scoring system). The expression of nucleoprotein NF-kappaB p65 was detected using Western blotting and the bronchoalveolar lavage fluid was collected to detect the total protein content and the levels of TNF-α and IL-1β.Results Compared with those in the S group, the Hofbauer scores, W/D, NF-kappaB p65 expression and BALF indexes including the total protein content and the levels of TNF-α and IL-1β in the M group and the R group were increased significantly(P<0.05). Compared with those in the M group, the Hofbauer scores, W/D, NF-kappaB p65 expression of lung tissue and BALF indexes in the R group were decreased significantly(P<0.05).Conclusion Resveratrol preconditioning can attenuate the acute lung injury induced by intestinal ischemia-reperfusion in rats. This effect may be related to inhibiting NF-kappaB activity in the lungs. |
| Key words: Resveratrol Reperfusion injury Acute lung injury Nuclear factor of kappa B(NF-kappaB) Cytokine |
