| 摘要: |
| ［摘要］ 目的 探讨负载血管基质片段（SVF）的甲基丙烯酸酐化明胶（GelMA）水凝胶用于促进皮下血管化乳头状软骨再生的可行性。方法 提取兔脂肪来源的SVF，体外条件下对SVF成分细胞进行培养，并检测其成脂、成骨及成软骨分化能力。随后将SVF均匀载入GelMA水凝胶中，并通过对比单纯GelMA水凝胶，验证SVF的载入对GelMA水凝胶可打印性的影响。随后分别将GelMA和GelMA/SVF复合水凝胶负载软骨细胞后植入裸鼠皮下培养4周，通过HE和Safranin-O染色对比两组水凝胶样本中软骨特异性细胞外基质再生情况，通过CD31免疫荧光染色对比两组水凝胶样本中血管化情况。结果 脂肪来源SVF成分细胞具有良好的增殖能力，并且具有向脂肪细胞、成骨细胞与软骨细胞分化的能力。GelMA/SVF复合水凝胶具有与GelMA水凝胶类似的3D打印性能。植入裸鼠皮下4周后，相较于GelMA水凝胶组，GelMA/SVF复合水凝胶组再生的软骨更加均质且丰厚，软骨特性生化成分（包括GAG和Collagen Ⅱ含量）表达量更高，且具有更好的血管化表达。结论 SVF的引入可以显著促进GelMA水凝胶实现皮下血管化乳头状软骨再生。 |
| 关键词: 乳头再造 软骨再生 血管基质片段 血管化 3D打印 |
| DOI：10.3969/j.issn.1674-3806.2022.06.06 |
| 分类号:R 655.8 |
| 基金项目:海南省重点研发项目（编号：ZDYF2020130） |
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| An experimental study on stromal vascular fraction-loaded GelMA hydrogel to promote subcutaneous vascularized nipple-shaped cartilage regeneration |
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ZHANG Yu, DING Jing-hao, CHEN Ru
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Department of Breast Surgery, Hainan General Hospital(Hainan Affiliated Hospital of Hainan Medical University), Haikou 570311, China
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| Abstract: |
| ［Abstract］ Objective To investigate the feasibility of stromal vascular fraction(SVF)-loaded methacrylate gelatin(GelMA) hydrogel in promoting the regeneration of subcutaneous vascularized nipple-shaped cartilage. Methods SVF derived from rabbit fat was extracted, and the component cells of SVF were cultured in vitro, and their adipogenic, osteogenic and chondrogenic differentiation abilities were detected. Then, SVF was uniformly loaded into GelMA hydrogel, and the influence of loaded SVF on the printability of GelMA hydrogel was verified by comparing with single GelMA hydrogel. Thereafter, GelMA and GelMA/SVF composite hydrogels were loaded with chondrocytes respectively, and were subcutaneously implanted into nude mice for 4 weeks. The regeneration of cartilage specific extracellular matrix in the two groups of hydrogel samples was compared by hematoxylin staining(HE) and Safranin-O staining, and the vascularization in the two groups of hydrogel samples was compared by CD31 immunofluorescence staining. Results Fat-derived SVF component cells had good proliferative ability, and could be differentiated into adipocytes, osteoblasts and chondrocytes. The GelMA/SVF composite hydrogel had comparable three-dimensional(3D) printing performance with single GelMA hydrogel. Four weeks after subcutaneous implantation in nude mice, the regenerated cartilage in the GelMA/SVF composite hydrogel group exhibited superior homogeneous and rich cartilage specific extracellular matrix deposition compared with that in the GelMA hydrogel group, and the expressions of cartilage specific biochemical components(including GAG and collagen Ⅱ contents) in the GelMA/SVF composite hydrogel group were higher than those in the GelMA hydrogel group, and the vascularized expression in the GelMA/SVF composite hydrogel group was better than that in the GelMA hydrogel group. Conclusion The introduction of SVF can significantly promote GelMA hydrogel to achieve subcutaneous vascularized nipple-shaped cartilage regeneration. |
| Key words: Nipple reconstruction Cartilage regeneration Stromal vascular fraction(SVF) Vascularization Three-dimensional(3D) printing |
