| 摘要: |
| ［摘要］ 目的 探讨新改良白膜法制备浓缩血小板（PC）的应用效果。方法 随机抽选2019年5月至2020年3月株洲市中心血站采集的400 ml规格全血220袋、300 ml规格全血30袋。比较不同离心程序、不同全血容量对PC质量的影响，以确定新改良白膜法的制备程序。比较富浆法、改良白膜法和新改良白膜法制备PC的质量。结果 全血离心方案6（全血离心：相对离心力1 993×g；白膜离心：相对离心力163×g）所得PC的血小板回收率最高；不同离心方案制备所得PC的红细胞混入量和白细胞混入量差异无统计学意义（P>0.05）。400 ml全血组的PC红细胞混入量显著高于300 ml全血组（P<0.05），白细胞混入量略高于300 ml全血组，但差异无统计学意义（P>0.05）。400 ml全血组的PC回收率高于300 ml全血组，差异有统计学意义（P<0.05）。新改良白膜法制备所得PC的白细胞混入量低于富浆法组和改良白膜法组，且血小板回收率更高，差异均有统计学意义（P<0.05）。结论 采用新改良白膜法制备PC具有较高血小板回收率，且红细胞、白细胞的混入量更低，值得推广。 |
| 关键词: 新改良白膜法 浓缩血小板 离心方案 全血容量 质量 |
| DOI：10.3969/j.issn.1674-3806.2022.08.09 |
| 分类号:R 331.1 |
| 基金项目:湖南省卫生健康委科研计划项目（编号：20201481） |
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| An exploration on the application effect of the new improved buffy coat method in the preparation of platelet concentrates |
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XIONG Zhi-gao, LIU Xin-chen, YANG Xiao-luo, et al.
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Zhuzhou Central Blood Station, Hunan 412007, China
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| Abstract: |
| ［Abstract］ Objective To explore the application effect of the new improved buffy coat method in the preparation of platelet concentrates(PC). Methods Two hundred and twenty bags of 400 ml whole blood and 30 bags of 300 ml whole blood collected at Zhuzhou Central Blood Station from May 2019 to March 2020 were randomly selected. The effects of different centrifugation procedures and different whole blood volumes on PC quality were compared to determine the preparation procedure of the new improved buffy coat method. The quality of PC prepared by the enriched plasma method, the improved buffy coat method and the new improved buffy coat method was compared. Results Whole blood centrifugation scheme 6(whole blood centrifugation: relative centrifugal force, 1 993×g; buffy coat centrifugation: relative centrifugal force, 163×g) had the highest platelet recovery rate. There were no significant differences in the mixed amount of red blood cells and the mixed amount of white blood cells of PC prepared by different centrifugation schemes(P>0.05). The mixed amount of red blood cells of PC in the 400 ml whole blood group was significantly higher than that in the 300 ml whole blood group(P<0.05), and the mixed amount of white blood cells in the 400 ml whole blood group was slightly higher than that in the 300 ml whole blood group, but the difference was not statistically significant(P>0.05). The recovery rate of PC in the 400 ml whole blood group was higher than that in the 300 ml whole blood group, and the difference was statistically significant(P<0.05). The amount of leukocytes mixed in PC prepared by the new improved buffy coat method was lower than that in the enriched plasma method group and the improved buffy coat method group, and the platelet recovery rate of the new improved buffy coat method was the highest, with statistically significant differences among the three groups(P<0.05). Conclusion The new improved buffy coat method to prepare PC has a higher platelet recovery rate, and less mixed amount of red blood cells and leukocytes, which is worthy of wide application. |
| Key words: New improved buffy coat method Platelet concentrates(PC) Centrifugation protocol Whole blood volume Quality |
