| 摘要: |
| ［摘要］ 分子诊断已成为病原体检测的重要手段。核酸扩增结合成簇规律间隔的短回文重复序列（CRISPR）的分子检测系统被认为是一种新一代即时分子诊断技术。CRISPR一管法是将核酸扩增和CRISPR检测集成于单一反应体系，备受研究者关注。近年来，基于多种生物传感器测量最终信号的核酸扩增-CRISPR/Cas一管法系统不断被开发，在病原体快速检测、食品安全和环境监测等领域具有应用潜力。该文就多种基于核酸扩增-CRISPR/Cas一管法生物传感器的研究进展作一综述。 |
| 关键词: 成簇规律间隔的短回文重复序列/Cas系统 一管法 生物传感器 分子诊断 |
| DOI：10.3969/j.issn.1674-3806.2026.09.21 |
| 分类号:R 446 |
| 基金项目:国家自然科学基金项目（编号：82270013，81870016）；广东省基础与应用基础研究基金项目（编号：2024A1515220116）；省部共建中亚高发病成因与防治国家重点实验室开放课题基金资助项目（编号：SKL-HIDCA-2024-GD6） |
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| Advances in biosensors based on nucleic acid amplification-CRISPR/Cas one-tube method |
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Ma Baoying1, Cai Xiangjing1, He Mianyun1, Liu Runbiao1, Lai Shiying1, Zhang Pengfei1, Xu Junfa1,2
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1.Institute of Laboratory Medicine, School of Medical Technology, Guangdong Medical University, Dongguan 523808, China; 2.Songshan Lake Innovation Center of Medicine & Engineering, Guangdong Medical University, Dongguan 523808, China
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| Abstract: |
| ［Abstract］ Molecular diagnostics has become an important means of pathogen detection. The nucleic acid amplification combined with clustered regularly interspaced short palindromic repeats(CRISPR) molecular detection system is regarded as a next-generation point-of-care molecular diagnostic technology. The CRISPR one-tube method integrates nucleic acid amplification and CRISPR detection into a single reaction system, which has attracted considerable attention from researchers. In recent years, nucleic acid amplification-CRISPR/CRISPR-associated proteins(Cas) one-tube method systems based on various biosensors for final signal measurement have been continuously developed, demonstrating potential applications in rapid pathogen detection, food safety, and environmental monitoring. In this paper, the advances in various biosensors based on nucleic acid amplification-CRISPR/Cas one-tube method are reviewed. |
| Key words: clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas) systems one-tube method biosensors molecular diagnostics |
