| 摘要: |
| ［摘要］ 目的 探讨山竹果皮提取物Garcinone E对鼻咽癌S18细胞迁移和侵袭的影响及相关机制。方法 使用不同浓度的Garcinone E处理S18细胞，通过CCK-8法检测细胞活力，通过Transwell实验检测S18细胞迁移、侵袭能力。通过Western blot实验检测S18细胞转移相关蛋白［组织金属蛋白酶抑制剂1(TIMP1)、组织金属蛋白酶抑制剂2(TIMP2)、基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)］、周期相关蛋白［细胞周期依赖性蛋白激酶抑制因子1A(p21)、周期蛋白依赖性激酶2(CDK2)、周期蛋白依赖性激酶6(CDK6)、周期蛋白依赖性激酶7(CDK7)、细胞周期蛋白B1(cyclin B1)］及自噬相关蛋白［微管相关蛋白1轻链3 β(LC3B)、泛素结合蛋白p62(SQSTM1/p62)、苄氯素1(beclin-1)、自噬相关蛋白3(Atg3)］的表达水平。结果 Garcinone E能够显著抑制S18细胞增殖（P<0.05），半抑制浓度（IC50）=（3.34±0.03）μmol/L，并对S18细胞的迁移和侵袭能力均具有抑制作用（P<0.05）。Garcinone E可以上调S18细胞的TIMP1、TIMP2、p21、LC3B、SQSTM1/p62、beclin-1和Atg3蛋白的表达水平（P<0.05），下调MMP-2、MMP-9、CDK2、CDK6、CDK7和cyclin B1蛋白的表达水平（P<0.05）。结论 Garcinone E能抑制鼻咽癌S18细胞的转移和侵袭能力，阻滞细胞周期，抑制细胞自噬，可作为鼻咽癌化疗药物研发的候选先导化合物。 |
| 关键词: Garcinone E 鼻咽癌 转移 细胞周期 自噬 |
| DOI：10.3969/j.issn.1674-3806.2023.04.08 |
| 分类号:R 739.6 |
| 基金项目:国家自然科学基金地区基金项目（编号：82260721，81660606）；国家自然科学基金青年基金项目（编号：81903644）；广西自然科学基金面上项目（编号：2018GXNSFAA281227）；2022年广西研究生教育创新计划项目（编号：YCSW2022215） |
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| A study on the effects of Garcinone E extracted from Mangostema mangostema peel on the migration and invasion of nasopharyngeal carcinoma S18 cells and their related mechanisms |
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WEI Dan, ZHANG Han, YIN Fu-qiang, et al.
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Key Laboratory of Longevity and Aging-Related Diseases of Chinese Ministry of Education, Center for Translational Medicine, Institute of Neuroscience and Guangxi Key Laboratory of Brain Science, Guangxi Medical University, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To investigate the effects of Garcinone E extracted from Mangostema mangostema peel on the migration and invasion of nasopharyngeal carcinoma S18 cells and their related mechanisms. Methods Different concentrations of Garcinone E were used to treat S18 cells. The cell viability was detected by CCK-8 method, and the migration and invasion ability of S18 cells were detected by Transwell assay. Western blot was used to detect the expression levels of transfer-related proteins［tissue inhibitor of metalloproteinase 1(TIMP1), tissue inhibitor of metalloproteinase 2(TIMP2), matrix metallopeptidase 2(MMP-2), matrix metallopeptidase 9(MMP-9)］, cyclin-related proteins［cyclin-dependent kinase inhibitor 1A(p21), cyclin-dependent kinase 2(CDK2), cyclin-dependent kinase 6(CDK6), cyclin-dependent kinase 7(CDK7), cyclin B1］ and autophagy-related proteins［microtubule-associated protein 1 light chain 3 beta(LC3B), ubiquitin-binding protein p62(SQSTM1/p62), beclin-1, autophagy-related 3(Atg3)］ in S18 cells. Results Garcinone E could significantly inhibit the proliferation of S18 cells(P<0.05), and its fifty percent inhibitory concentration(IC50)=(3.34±0.03)μmol/L, and could inhibit the migration and invasion ability of S18 cells(P<0.05). Garcinone E could up-regulate the expression levels of TIMP1, TIMP2, p21, LC3B, SQSTM1/p62, beclin-1 and Atg3 proteins in S18 cells(P<0.05), and could down-regulate the expression levels of MMP-2, MMP-9, CDK2, CDK6, CDK7 and cyclin B1(P<0.05). Conclusion Garcinone E can inhibit the metastasis and invasion ability of nasopharyngeal carcinoma S18 cells, block cell cycle and inhibit autophagy, and can be used as a candidate lead compound for the development of chemotherapy drug for nasopharyngeal carcinoma. |
| Key words: Garcinone E Nasopharyngeal carcinoma Metastasis Cell cycle Autophagy |
