| 摘要: |
| ［摘要］ 目的 分析帕金森病伴抑郁（DPD）患者肠道菌群的变化，并分析其代谢功能。方法 选择2021年6月至2022年10月广东省第二人民医院神经内科收治的DPD患者48例（DPD组），帕金森病不伴抑郁（NDPD）患者47例（NDPD组），选入帕金森病(PD)患者的健康配偶43名作为对照组。采集研究对象粪便样本，提取样本中的细菌DNA进行16SrDNA测序，比较三组间肠道菌群结构差异，分析DPD组差异菌属。应用PICRUSt2软件对肠道菌群测序结果进行代谢功能分析。结果 与对照组相比，DPD组和NDPD组的菌群α多样性显著增高（P<0.05）。β多样性分析结果显示，DPD组和NDPD组肠道菌群构成差异有统计学意义（P<0.05）。相较于对照组和NDPD组，DPD组放线菌门（Actinobacteria）、互养菌门（Synergistetes）丰度增加，与对照组比较差异有统计学意义（P<0.05），但与NDPD组比较差异无统计学意义（P>0.05）。线性判别分析（LDA）结果显示，另枝菌属（Alistipes）、Anaerotruncus属、Dielma属、霍尔德曼氏菌属（Holdemania）、芽胞杆菌属（Cloacibacillus）、柯林斯氏菌属（Collinsella）是DPD患者的特征性肠道菌群。PICRUSt2代谢功能分析发现，光合生物中的碳固定和抗坏血酸抵抗是DPD患者肠道菌群的主要代谢途径。结论 DPD患者肠道菌群与NDPD患者及健康者相比存在显著差异，DPD患者特征性肠道菌群可能通过蛋白酰化、抗坏血酸抵抗途径参与疾病的发病过程。 |
| 关键词: 帕金森病 抑郁 肠道菌群 高通量测序 代谢功能 |
| DOI：10.3969/j.issn.1674-3806.2023.04.09 |
| 分类号:R 741.02 |
| 基金项目:国家重点研发计划项目（编号：2021YFC2009400）；广东省重点领域研发计划项目（编号：2020B0101130020）；广东省科技创新战略专项资金项目（编号：2018A030313649）；广东省第二人民医院青年科研基金项目（编号：YQ2020-009） |
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| Changes of gut microbiota and its metabolic function analysis in patients with Parkinson′s disease and depression |
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REN Teng-zhu, LI Gui-hua, LIANG Xiao-yu, et al.
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Department of Neurology, Guangdong Second Provincial General Hospital, Guangzhou 510310, China
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| Abstract: |
| ［Abstract］ Objective To analyze the changes of gut microbiota and to analyze its metabolic function in patients with Parkinson′s disease and depression(DPD). Methods Forty-eight patients with DPD(the DPD group) and 47 patients with Parkinson′s disease but without depression(the NDPD group) who were admitted to the Department of Neurology of Guangdong Second Provincial General Hospital from June 2021 to October 2022 were selected. Forty-three healthy spouses of the Parkinson′s disease(PD) patients were selected as the control group. Fecal samples of the research subjects were collected, and the bacterial deoxyribonucleic acid(DNA) in the samples was extracted for 16SrDNA sequencing. The structural differences of the gut microbiota were compared among the three groups, and the different bacterial genus in the DPD group were analyzed. PICRUSt2 software was used to analyze the metabolic function of the gut microbiota sequencing results. Results Compared with that in the control group, the α diversities of microflora in the DPD group and the NDPD group were significantly increased(P<0.05). The results of β diversity analysis showed that the difference in the composition of gut microbiota between the DPD group and the NDPD group was statistically significant(P<0.05). Compared with that in the control group and the NDPD group, the abundance of Actinobacteria and Synergistetes in the DPD group was increased. There were significant differences in the increased abundances of Actinobacteria and Synergistetes between the DPD group and the control group(P<0.05), but there was no significant difference between the DPD group and the NDPD group(P>0.05). The results of linear discriminant analysis(LDA) showed that Alistipes, Anaerotruncus, Dielma, Holdemania, Cloacibacillus and Collinsella were the characteristic gut microbiota of the DPD patients. The results of PICRUSt2 metabolic function analysis showed that carbon fixation in photosynthetic organism and ascorbic acid resistance were the main metabolic pathways of the gut microbiota of the DPD patients. Conclusion The gut microbiota of the DPD patients is significantly different from that of the NDPD patients and the healthy people. The characteristic gut microbiota of the DPD patients may participate in the pathogenesis of the diseases through the pathways of protein acylation and ascorbic acid resistance. |
| Key words: Parkinson′s disease(PD) Depression Gut microbiota High throughput sequencing Metabolic function |
