| 摘要: |
| ［摘要］ 目的 探讨血清8-羟基脱氧鸟苷（8-OHdG）、基质细胞衍生因子-1（SDF-1）在新生儿呼吸窘迫综合征（NRDS）患儿中的表达及临床意义。方法 招募2021年7月至2024年5月商洛市中心医院收治的NRDS患儿108例（NRDS组）和同期正常新生儿52名（对照组）。NRDS患儿根据病情分为轻度NRDS组（22例）、中度NRDS组（50例）和重度NRDS组（36例）。采用酶联免疫吸附试验法检测血清8-OHdG、SDF-1水平。采用Cox回归分析NRDS患儿预后的影响因素。通过受试者工作特征（ROC）曲线分析8-OHdG、SDF-1水平对NRDS患儿死亡的预测效能。结果 NRDS组8-OHdG水平高于对照组［（13.81±3.90）ng/mL vs （3.70±1.47）ng/mL］，SDF-1水平高于对照组［（12.46±1.62）ng/mL vs （8.23±1.32）ng/mL］，差异有统计学意义（P<0.05）。轻度NRDS组、中度NRDS组、重度NRDS组8-OHdG、SDF-1水平依次升高（P<0.05）。108例NRDS患儿入院后28 d内死亡率为25.00%（27/108）。多因素Cox回归分析结果显示，重度NRDS以及较高的8-OHdG、SDF-1水平是NRDS患儿发生死亡的独立危险因素（P<0.05）。ROC曲线分析结果显示，8-OHdG、SDF-1水平均有助于预测NRDS患儿死亡（P<0.05），两者联合的预测效能［AUC（95%CI）=0.925（0.859～0.967）］较单一指标更高（P<0.05），灵敏度为92.59%，特异度为77.78%。结论 血清8-OHdG、SDF-1水平升高与NRDS患儿病情分度及死亡风险增加密切相关，两者联合预测NRDS患儿预后的效能较单一指标高。 |
| 关键词: 新生儿呼吸窘迫综合征 8-羟基脱氧鸟苷 基质细胞衍生因子-1 病情分度 预后 |
| DOI：10.3969/j.issn.1674-3806.2025.07.14 |
| 分类号: |
| 基金项目:陕西省卫生健康科研基金项目（编号：2022D051） |
|
| Expressions of serum 8-OHdG and SDF-1 in pediatric patients with neonatal respiratory distress syndrome and their clinical significance |
|
YANG Lijun, SUN Xu′e, CHEN Tingting
|
|
Department of Neonatology, Shangluo Central Hospital, Shangluo 726000, China
|
| Abstract: |
| ［Abstract］ Objective To explore the expressions of serum 8-hydroxy-2′-deoxyguanosine(8-OHdG) and stromal cell-derived factor-1(SDF-1) in pediatric patients with neonatal respiratory distress syndrome(NRDS) and their clinical significance. Methods A total of 108 pediatric patients with NRDS(NRDS group) and 52 normal neonates(control group) who were admitted to Shangluo Central Hospital from July 2021 to May 2024 were recruited. The pediatric patients with NRDS were divided into mild NRDS group(22 patients), moderate NRDS group(50 patients) and severe NRDS group(36 patients) according to their disease severity. Serum 8-OHdG and SDF-1 levels were detected by using enzyme-linked immunosorbent assay. Cox regression was used to analyze the influencing factors of prognosis in the pediatric patients with NRDS. The efficacy of 8-OHdG and SDF-1 levels in predicting death of the pediatric patients with NRDS was analyzed by using receiver operating characteristic(ROC) curve. Results The level of 8-OHdG in the NRDS group was higher than that in the control group［(13.81±3.90)ng/mL vs (3.70±1.47)ng/mL］, and the level of SDF-1 in the NRDS group was higher than that in the control group［(12.46±1.62)ng/mL vs (8.23±1.32)ng/mL］, with statistically significant differences between the two groups(P<0.05). The levels of 8-OHdG and SDF-1 in the mild NRDS group, the moderate NRDS group and the severe NRDS group were gradually increased(P<0.05). The mortality rate of the 108 pediatric patients with NRDS within 28 days after admission was 25.00%(27/108). The results of multivariate Cox regression analysis showed that severe NRDS, and higher 8-OHdG and SDF-1 levels were independent risk factors for death in the pediatric patients with NRDS(P<0.05). The results of ROC curve analysis showed that the levels of 8-OHdG and SDF-1 were helpful for predicting death of the pediatric patients with NRDS(P<0.05). The predictive efficacy of the combination of the two indicators［AUC(95%CI)=0.925(0.859-0.967)］ was higher than that of a single indicator(P<0.05), with a sensitivity of 92.59 % and a specificity of 77.78 %. Conclusion The elevated serum 8-OHdG and SDF-1 levels are closely related to the disease severity and the increase of death risk in pediatric patients with NRDS. The combination of the two indicators is more effective than a single indicator in predicting prognosis of the pediatric patients with NRDS. |
| Key words: Neonatal respiratory distress syndrome(NRDS) 8-Hydroxy-2′-deoxyguanosine(8-OHdG) Stromal cell-derived factor-1(SDF-1) Disease severity Prognosis |
