| 摘要: |
| ［摘要］ 目的 探讨驱动蛋白家族成员18B（KIF18B）、抗沉默功能蛋白1B（ASF1B）在子宫内膜癌（EC）组织中的表达情况，分析二者与临床病理特征和生存预后的关系。方法 通过生物信息学方法分析正常组织和子宫体子宫内膜癌（UCEC）组织中KIF18B、ASF1B mRNA和蛋白的表达水平。收集2016年7月至2021年7月西安市人民医院（西安市第四医院）收治的226例EC患者的临床资料。术中留取EC组织和癌旁组织（距癌组织边缘3～5 cm）。采用实时荧光定量聚合酶链反应（FQ-PCR）法检测EC组织和癌旁组织KIF18B mRNA、ASF1B mRNA的表达水平。采用SP法免疫组化染色检测EC组织和癌旁组织中KIF18B、ASF1B蛋白的表达情况。术后对患者进行3年随访，记录患者生存预后情况。结果 TCGA数据库显示，UCEC组织中KIF18B mRNA、ASF1B mRNA水平显著高于正常组织（P<0.05）。CPTAC数据库显示，UCEC组织中ASF1B蛋白表达水平显著高于正常组织（P<0.05）。EC组织中KIF18B mRNA、ASF1B mRNA水平显著高于癌旁组织（P<0.05）。EC组织中KIF18B蛋白阳性表达率、ASF1B蛋白阳性表达率显著高于癌旁组织（P<0.05）。KIF18B、ASF1B蛋白表达情况与肌层浸润深度、国际妇产科联盟（FIGO）分期、淋巴结转移情况有关（P<0.05）。多因素Cox回归分析结果显示，FIGO分期Ⅲ期、KIF18B蛋白阳性表达、ASF1B蛋白阳性表达是EC患者发生死亡的独立危险因素（P<0.05）。KIF18B、ASF1B蛋白阴性表达患者的生存预后显著优于其阳性表达患者（log-rank检验： χ2=8.518，P=0.004；log-rank检验： χ2=9.993，P=0.002）。结论 EC组织中KIF18B、ASF1B均呈高表达，且二者与肌层浸润深度、FIGO分期、淋巴结转移情况和生存预后密切相关。 |
| 关键词: 子宫内膜癌 驱动蛋白家族成员18B 抗沉默功能蛋白1B 临床病理特征 生存预后 |
| DOI：10.3969/j.issn.1674-3806.2025.09.13 |
| 分类号:R 737.33 |
| 基金项目:陕西省重点研发计划项目（编号：2023-YBSF-654） |
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| Association of expressions of KIF18B and ASF1B in endometrial carcinoma tissues with clinicopathological characteristics and survival prognosis in endometrial carcinoma patients |
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YE Xiaolin, LIANG Xinli, ZHANG Chao
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Department of Obstetrics and Gynecology, Xi′an People′s Hospital(Xi′an Fourth Hospital), Xi′an 710000, China
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| Abstract: |
| ［Abstract］ Objective To explore the expressions of kinesin family member 18B(KIF18B) and anti-silencing function 1B(ASF1B) in endometrial carcinoma(EC) tissues, and to analyze the association of the expressions of KIF18B and ASF1B in endometrial carcinoma tissues with clinicopathological characteristics and survival prognosis. Methods The expression levels of KIF18B, ASF1B mRNA and protein in normal tissues and uterine corpus endometrial carcinoma(UCEC) tissues were analyzed by using bioinformatics methods. The clinical data of 226 patients with EC who were admitted to Xi′an People′s Hospital(Xi′an Fourth Hospital) from July 2016 to July 2021 were collected. During the operation, the EC tissues and their adjacent tissues(3 to 5 cm away from the edge of the EC tissues) of the EC patients were collected. The expression levels of KIF18B mRNA and ASF1B mRNA in the EC tissues and their adjacent tissues were detected by using real-time fluorescent quantitative polymerase chain reaction(FQ-PCR). The expressions of KIF18B and ASF1B proteins in the EC tissues and their adjacent tissues were detected by using immunohistochemical staining(SP method). After the operation, the patients were followed up for 3 years and their survival prognosis was recorded. Results The Cancer Genome Atlas(TCGA) database showed that the levels of KIF18B mRNA and ASF1B mRNA in the UCEC tissues were significantly higher than those in the normal tissues(P<0.05). Clinical Proteomic Tumor Analysis Consortium(CPTAC) database showed that the expression level of ASF1B protein in the UCEC tissues was significantly higher than that in the normal tissues(P<0.05). The levels of KIF18B mRNA and ASF1B mRNA in the EC tissues were significantly higher than those in their adjacent tissues(P<0.05). The positive expression rates of KIF18B protein and ASF1B protein in the EC tissues were significantly higher than those in their adjacent tissues(P<0.05). The expressions of KIF18B and ASF1B proteins were associated with the depth of myometrial infiltration, International Federation of Gynecology and Obstetrics(FIGO) stage and lymph node metastasis(P<0.05). The results of multivariate Cox regression analysis showed that FIGO stage Ⅲ, positive expression of KIF18B protein, and positive expression of ASF1B protein were independent risk factors for death in the EC patients(P<0.05). The survival prognosis of the patients with negative expressions of KIF18B and ASF1B proteins was significantly better than that of the patients with positive expressions of KIF18B and ASF1B proteins(log-rank test: χ2=8.518, P=0.004; log-rank test: χ2=9.993, P=0.002). Conclusion In EC tissues, both KIF18B and ASF1B are highly expressed, and both are closely associated with the depth of myometrial infiltration, FIGO stage, lymph node metastasis and survival prognosis. |
| Key words: Endometrial carcinoma(EC) Kinesin family member 18B(KIF18B) Anti-silencing function 1B(ASF1B) Clinicopathological characteristics Survival prognosis |
