| 摘要: |
| ［摘要］ 目的 从CM10、IMAC3-Cu这两种芯片中选择适合应用于血清蛋白质指纹图谱研究的芯片种类。方法 选择慢性乙型肝炎患者、肝硬化患者和健康人血清各13例，采用CM10、IMAC3-Cu两种蛋白芯片，经SELDI-TOF-MS技术对样本进行检测比较。结果 在2000-50000Da范围内， CM10和IMAC3-Cu芯片在各实验组捕获的有效蛋白峰数的差异均有统计学意义（P<0.05）。结论 CM10能得到更多慢性乙型肝炎患者、肝硬化患者和健康人的有意义的蛋白峰值，能更好的应用于血清蛋白组学的实验研究。 |
| 关键词: 蛋白质组学 表面增强激光解吸/电离时间飞行质谱 蛋白质芯片 |
| DOI：10.3969/j.issn.1674-3806.2009.04.004 |
| 分类号:Q 753 |
| 基金项目:广西医疗卫生重点科研课题（桂卫重200627） |
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| Comparative analysis of two kinds of protein chips on screen of serum protein markers |
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XU Ming-hui, DENG Jing-huan, LI Shan, et al.
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The Clinic Laboratory Department of the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China
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| Abstract: |
| ［Abstract］ Objective To select a suitable protein chip for detection of serum protein fingerprint from two kinds of chips(CM10 and IMAC3-Cu). Methods Serum samples from 13 patients with chronic hepatitis B, 13 patients with liver cirrhosis and 13 normal control people were collected respectively, peptide spectra were determined by SELDI-TOF-MS measurement. Results In the range from 2000Da to 50000Da, the number of significant protein peaks captured in each experimental group has a significant difference between CM10 and IMAC3-Cu chips（P<0.05）.Conclusion CM10 chips can detect more significant difference protein peaks compared with IMAC3-Cu chips and preferably apply to the research of the serum protein fingerprints. |
| Key words: Proteomics Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry Protein chip |
