| 摘要: |
| ［摘要］ 目的 探讨人骨髓间充质干细胞体外长期传代培养、扩增及其生物学特性，观察其恶性转化趋势的可能性。方法 采用密度梯度离心差速贴壁法对人骨髓来源的间充质干细胞进行分离纯化，并进行体外长期传代，观察细胞形态及生长方式；鉴定细胞分化能力；相关癌标志P53、Ki67的表达与成瘤性检测。结果 经过长期体外培养的人间充质干细胞，至36代时，细胞逐渐老化；成骨分化诱导能力丧失；相关癌标志表达阴性；无成瘤性。结论 人骨髓间充质干细胞在体外可以有限传代，无显著恶性转化趋势。 |
| 关键词: 人骨髓间充质干细胞 细胞培养 恶性转化 |
| DOI：10.3969/j.issn.1674-3806.2009.06.03 |
| 分类号:Q 343 |
| 基金项目:广西自然科学青年基金项目（青0447026），广西留学回国人员科学基金项目（桂科回0731016） |
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| Investigation of long term in vitro culture and malignant transformation of human mesenchymal stem cell |
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HUANG Ying-hua, WEI Ying, CAI Jie, et al.
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Department of Pathophysiology, Guangxi Medical University, Nanning 530021, China
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| Abstract: |
| ［Abstract］ Objective To discuss the biological properties of human bone marrow derived mesenchymal stem cell (hBMSC) after long term in vitro culture and it′s malignant transformation.Methods hBMSCs were isolated and enriched by the gradient centrifugation protocol and cultured in vitro for long term. During in vitro culture, the cell line′s morphology were observed; It′s differentiation were evaluated; The expression of cancer relative markers: P53、Ki67 were detectived, and the tumor-initiate ability were assessed.Results When culture to the 36th passage, hMSC were gradually aging, cannot differentiate to ossification, cancer relative markers were still negative, there was no tumor initiation.Conclusion hBMSCs can be cultured in vitro for limited time, there was no significantly malignant transformation during the limited term. |
| Key words: Human bone marrow derived mesenchymal stem cell Cell culture Malignant transformation |
